Dietary protein deficiency affects n?3 and n?6 polyunsaturated fatty acids hepatic storage and very low density lipoprotein transport in rats on different diets |
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Authors: | Mahmoud Bouziane Josiane Prost Jacques Belleville |
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Affiliation: | (1) Unité de Recherches de Nutrition Cellulaire et Métabolique, Université de Bourgogne, Faculté des Sciences Mirande, BP 138, 21004 Dijon Cedex, France |
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Abstract: | Fatty livers and the similarity between the skin lesions in kwashiorkor and those described in experimental essential fatty
acid (EFA) deficiency have led to the hypothesis that protein and EFA deficiencies may both occur in chronic malnutrition.
The relationship between serum very low density lipoprotein (VLDL) and hepatic lipid composition was studied after 28 d of
protein depletion to determine the interactions between dietary protein levels and EFA availability. Rats were fed purified
diets containing 20 or 2% casein and 5% fat as either soybean oil rich in EFA, or salmon oil rich in eicosapentaenoic (EPA)
and docosahexaenoic (DHA) acids, or hydrogenated coconut, oil poor in EFA. Animals were divided into six groups, SOC (20%
casein +5% soybean oil), SOd (2% casein +5% soybean oil), COC (20% casein +5% hydrogenated coconut oil), COd (2% casein +
5% hydrogenated coconut oil), SAC (20% casein +5% salmon oil) and SAd (2% casein +5% salmon oil). After 28 d, liver steatosis
and reduced VLDL-phospholipid contents (P<0.001) were observed in protein-deficient rats. In protein deficiency, triacylglycerol and phospholipid fatty acid compositions
in both liver and VLDL showed a decreased polyunsaturated-to-saturated fatty acid ratio. This ratio was higher with the salmon
oil diets and lower with the hydrogenated coconut oil diets. Furthermore, independent of the oil in the diet, protein deficiency
decreased linoleic and arachidonic acids in VLDL phospholipids. Conversely, despite decreased proportions of EPA at low protein
levels, DHA levels remained higher in rats fed salmon oil diets. While in rats fed the hydrogenated coconut oil-fed diets
the amount of 22∶5n−6 was lower in liver, it was higher in VLDL lipids at low protein levels. Both EPA and arachidonic acid
are precursors of eicosanoids and their diminution may be related to certain clinical symptoms seen in infants suffering from
kwashiorkor. |
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