| 人尿中Carphedone的气相色谱-质谱分析 |
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| 引用本文： | 崔凯荣,张亦农,刘欣. 人尿中Carphedone的气相色谱-质谱分析[J]. 质谱学报, 2004, 25(3): 166-166 |
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| 作者姓名： | 崔凯荣 张亦农 刘欣 |
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| 作者单位： | 国家体育总局运动医学研究所,北京,100029 |
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| 摘 要： | 建立检测人尿中兴奋剂 Carphedone分析方法。 5 0 m g尿样流经 XAD-2填装小柱淋洗 ,用甲醇洗脱后收集洗脱液 ,经过缓冲和酶解后振荡、离心 ,加入衍生化试剂 N-甲基 -N-三甲硅烷基三氟乙酰胺 ( MSTFA)和三甲硅烷基咪唑 ( TMSI)进行 TMS硅烷基化。气相色谱 -质谱 ( GC/MS)分析结果表明 :可以检测到 5 0 h内的代谢产物 ,同时可以检测 Carphedone衍生化后的脱水反应产物。此方法适用于兴奋剂检测尿中Carphedone代谢物的分析
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| 关 键 词： | 质谱学 Carphedone测定 气相色谱-质谱(GC/MS) 尿液 三甲硅烷基(TMS) |
| 文章编号： | 1004-2997(2004)03-166-03 |
| 收稿时间： | 2003-12-08 |
| 修稿时间： | 2003-12-08 |
Detection of Carphedone in Human Urine by Gas Chromatography-Mass Spectrometry |
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| CUI Kai-rong,ZHANG Yi-nong,LIU Xin. Detection of Carphedone in Human Urine by Gas Chromatography-Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2004, 25(3): 166-166 |
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| Authors: | CUI Kai-rong ZHANG Yi-nong LIU Xin |
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| Abstract: | Carphedone(2-Oxo-4-phenylpyrrolidine acetamide) as a new stimulant has been banned by International Olympic Committee(IOC) since 1997. After administration of single dose of the drug,the 50mg of urine samples were extracted by use of XAD packed minicolumn and eluted with methanol. The concentrate solution was collected, buffered, enzymed, centrifuged and derivated with N-methyl-N-trimethylsilyl trifluoroacetamide(MSTFA) under the condition of procedure for anabolic agents as the routine method in the lab. Carphedone itself was detected by gas chromatography-mass spectrometry(GC/MS). The results showed the metabolization products within 50 hours and dehydrated products of the molecule are found and recognized to be formed during derivatization. |
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| Keywords: | mass spectrometry detection of Carphedone gas chromatography-mass spectrometry(GC/MS) urine trimethylsilyl(TMS) |
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