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| TaERFL1基因过表达提高转基因烟草耐盐性 | |
| 引用本文： | 郭恒新,徐兆师,李连城,陈明,刘丽,陈学平,马有志. TaERFL1基因过表达提高转基因烟草耐盐性[J]. 中国烟草学报, 2011, 17(2): 75-78. DOI: 10.3969/j.issn.1004-5708.2011.02.016 |
| 作者姓名： | 郭恒新 徐兆师 李连城 陈明 刘丽 陈学平 马有志 |
| 作者单位： | 1. 中国科学技术大学研究生院,合肥市金寨路96号230026;中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物遗传育种重点开放实验室,北京100081 2. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物遗传育种重点开放实验室,北京,100081 3. 中国科学技术大学研究生院,合肥市金寨路96号,230026 |
| 基金项目： | 国家自然科学基金，转基因生物新品种培育重大专项 |
| 摘 要： | 将转录因子ERFⅣ家族基因TaERFL1构建在由组成型CaMV35S强启动子控制的双子叶转化载体pBI121上,通过农杆菌介导法将其转入烟草新华1号.以转TaERFL1基因烟草为材料,研究ERF因对烟草耐盐性的影响,并测定盐胁迫下转基因烟草的叶绿素含量.结果显示,在高盐胁迫下,TaERFL1基因的过表达促进了转基因烟草... |
| 关 键 词： | 小麦 ERF 转基因 耐盐性 烟草 |
Overexpression of TaERFL1 gene increases salt tolerance in transgenic tobacco plants |
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| GUO Heng-xin,XU Zhao-shi,LI Lian-cheng,CHEN Ming,LIU Li,CHEN Xue-ping,MA You-zhi. Overexpression of TaERFL1 gene increases salt tolerance in transgenic tobacco plants[J]. Acta Tabacaria Sinica, 2011, 17(2): 75-78. DOI: 10.3969/j.issn.1004-5708.2011.02.016 | |
| Authors: | GUO Heng-xin XU Zhao-shi LI Lian-cheng CHEN Ming LIU Li CHEN Xue-ping MA You-zhi |
| Affiliation: | GUO Heng-xin1,2,XU Zhao-shi2,LI Lian-cheng2,CHEN Ming2,LIU Li2,CHEN Xue-ping1,MA You-zhi21 Graduate School of University of Science and Technology of China,Anhui 230026,China,2 Institute of Crop Science,Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources andGenetic Improvement,NFCRI/Key Laboratory of Crop Genetics and Breeding,Ministry of Agriculture,Beijing 100081 |
| Abstract: | TaERFL1, which belongs to ERF IV subfamily,was induced in wheat by abiotic and biotic stresses.Transgenic vector(pBI121::TaERFL1) was constructed, which contained CaMV35S promoter.Transgenic tobacco plants were obtained by Agrobacterium-mediated transformation.Salt-tolerance and chlorophyll content in transgenic tobacco plant were investigated.Results showed that overexpression of TaERFL1 promoted the development of roots and growth of plants,and increased chlorophyll content under salt environments.Overexp... |
| Keywords: | wheat ERF transgenic plants salt tolerance tobacco plants |
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