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| 湖北省烟草黑胫病菌生理小种研究初报 | |
| 引用本文： | 李锡坤,孔凡玉,李锡宏,王静,张成省,冯超,陈雅琼,刘璇,苏振刚,李佰乐. 湖北省烟草黑胫病菌生理小种研究初报[J]. 中国烟草科学, 2011, 32(3): 84-88. DOI: 10.3969/j.issn.1007-5119.2011.03.018 |
| 作者姓名： | 李锡坤 孔凡玉 李锡宏 王静 张成省 冯超 陈雅琼 刘璇 苏振刚 李佰乐 |
| 作者单位： | 1. 中国农业科学院烟草研究所,青岛,266101;中国农业科学院研究生院,北京 100081 2. 中国农业科学院烟草研究所,青岛,266101 3. 湖北省烟草研究所,武汉,430000 4. 云南省玉溪市烟草公司,云南,玉溪,653100 |
| 摘 要： | 在湖北省襄樊、恩施等主要烟区采集烟草黑胫病病株样本111个,分离纯化出30个烟草黑胫病菌株。采用游动孢子悬浮液注射接种鉴别寄主和TTZ(2,3,5-氯化三苯基四氮唑)颜色反应两种方法鉴定其生理小种类型。结果表明,供试的30个菌株在TTZ培养基中的反应皆为红色,有24个菌株在鉴别寄主反应中对L8和NC1071有较强致病力,而对N.nesophila无致病力,应为1号生理小种,占供试菌系的80%,为湖北省优势生理小种;其他6个菌株中有4个菌株对NC1071有较强致病力,但对L8致病力较弱,有1个菌株对L8和NC1071致病力较弱;1个菌株对NC1071致病力较弱,但对L8有较强致病力;6个菌株对N.nesophila无致病力,是否为2号生理小种还是其他致病型,尚待进一步研究确定。 |
| 关 键 词： | 烟草 黑胫病菌 生理小种 湖北 |
Preliminary Report on Physiological Race of Phytophthora parasitica in Hubei |
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| LI Xikun,KONG Fanyu,LI Xihong,WANG Jing,ZHANG Chengsheng,FENG chao,CHENYaqiong,LIU Xuan,SU Zhengang,LI Baile. Preliminary Report on Physiological Race of Phytophthora parasitica in Hubei[J]. Chinese Tobacco Science, 2011, 32(3): 84-88. DOI: 10.3969/j.issn.1007-5119.2011.03.018 | |
| Authors: | LI Xikun KONG Fanyu LI Xihong WANG Jing ZHANG Chengsheng FENG chao CHENYaqiong LIU Xuan SU Zhengang LI Baile |
| Affiliation: | LI Xikun1,2,KONG Fanyu1,LI Xihong3,WANG Jing1,ZHANG Chengsheng1,FENG chao1,CHENYaqiong1,LIU Xuan1,SU Zhengang1,LI Baile4(1.Tobacco Research Institute of CAAS,Qingdao 266101,China,2.Graduate School of CAAS,Beijing 100081,3.Tobacco Research Institute of Hubei,Wuhan,430000,4.YUXI Tobacco 653100) |
| Abstract: | The physiological races of 30 Phytophthora parasitica var.nicotianae strains were isolated from 111 samples in Xiangfan,Enshi and other major tobacco area of Hubei,and identified by zoospore suspension inoculation and TTZ(2,3,5-tripheny ltetrazolium chloride) color reaction.The results showed that all these 30 strains were presented red reaction in TTZ medium.And according to the reaction on the differential host,24 strains belonged to physiological race 1 and were the Dominant race,which take up 80 percent... |
| Keywords: | tobacco phytophthora parasitica var.nicotianae physiological race Hubei |
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