顶空固相微萃取法分析刺楸树杆和树皮中挥发油的化学成分

应用顶空固相微萃取法(HS-SPME)对刺楸树杆和树皮中的挥发油成分进行提取,通过气相色谱-质谱法与Kovats色谱保留指数相结合进行定性定量分析,以峰面积归一化法计算各组分的相对含量。结果表明从刺楸树杆和树皮中分别鉴定出53种和67种化合物,分别占挥发油总峰面积的95.91%和96.60%,其中树杆和树皮中主要成分均为γ-榄香烯(18.59%,14.60%),大根香叶烯d(19.09%,15.79%),(E,E)-β-金合欢烯(13.94%,16.88%)。

Analysis of Chemical Compositions of Volatile oil in Kalopanax septemlobus (Thunb.) Koidz. Trunk and Bark by Headspace Solid-phase Micro-extraction

The volatile compounds were extracted in the trunk and bark of Kalopanax septemlobus(Thunb.) Koidz. by headspace solid-phase micro-extraction(HS-SPME). Their constituents were analyzed and identified by gas chromatography-mass spectrometry combined with Kovats retention index,and the relative contents were quantified by calculating basing on the peak areas norma-lization method. The results showed that 53 volatile constituents in the trunk and 67 volatile constituents in the bark of K.septemlobus were identified, accounting for 95.91% and 96.60% of the total peak areas, respectively.The main components obtained from trunk and bark were found to be quite similar. γ-elemene(18.59%,14.60%), germacrene d (19.09%,15.79%), trans-β-farnesene (13.94%,16.88%).