荞麦过敏原致敏蛋白组分研究

目的对荞麦过敏原的主要致敏蛋白进行研究。方法收集26例荞麦过敏症患者,详细询问临床病史,留取血清建立血清库;荧光免疫分析仪ImmunoCAP系统检测荞麦特异性IgE水平;用免疫印迹法测定每种致敏蛋白组分的灰度值。结果共收集荞麦过敏患者26例(男10例、女16例,平均年龄29岁)。荞麦特异性IgE的中位数水平是8.385 KUA/L。免疫印迹法识别了荞麦过敏原中的15种致敏蛋白:10 kDa(8%)、13kDa(4%)、14 kDa(12%)、16 kDa(4%)、18~19 kDa(100%)、22~24 kDa(46%)、34 kDa(31%)、36 kDa(58%)、38kDa(15%)、40~50 kDa(46%)、54 kDa(23%)、56 kDa(19%)、69~70 kDa(46%)、100~130 kDa(27%)和130~170kDa(19%)。结论荞麦中的18~19、22~24、36、40~50和69~70 kDa蛋白显示高阳性率,可能为荞麦过敏原的主要致敏蛋白。

Study on allergens of buckwheat allergy

Objective To investigate the most related allergen components in buckwheat allergen. Methods Twenty six patients with buckwheat allergy were enrolled, their detail clinical history was inquired and a serum library was established. Buckwheat specificIgE was checked by the immunoCAP system and immunoblotting was performed to measure intensity of each component. Results Twenty-six patients (10 males and 16 females) were included in this study (average age was 29 years old). The median of buckwheat sIgE was 8.385 KUA/L. Fifteen allergens were identified by immunoblotting: 10 kDa(8%), 13 kDa(4%), 14 kDa(12%), 16 kDa(4%), 18~19 kDa(100%), 22~24 kDa(46%), 34 kDa(31%), 36 kDa(58%), 38 kDa(15%), 40~50 kDa(46%), 54 kDa(23%), 56 kDa(19%), 69~70 kDa(46%), 100~130 kDa(27%) and 130~170 kDa(19%) in the buckwheat proteins. Conclusion The 18~19, 22~24, 36, 40~50 and 69~70 kDa proteins in buckwheat are implicated as major allergens with high positive rates relatively.