液相色谱-串联四极杆飞行时间质谱快速筛查食品中违禁着色剂

目的建立一种快速筛查食品中违禁着色剂的液相色谱-串联四极杆飞行时间质谱(liquid chromatography-tandem quadrupole time of flight mass spectrometry,LC-QTOF-MS)检测方法。方法收集102种着色剂名称、CAS号、中性分子式、结构式等信息并录入到PCDL软件中。配制相应着色剂溶液后,在MS模式下采集一级谱图数据。确认一级质谱数据后,在Targeted MS/MS模式下给予目标离子10~60 e V的碰撞能,获取二级谱图数据。结果本研究建立了102种着色剂(其中可食用色素16种、违禁工业染料86种)的一级和二级质谱谱库,同时分离了8组相近中性分子量着色剂。结论该方法快速、准确、灵敏,适合筛查食品中未知违禁添加的着色剂并可以快速分离定量。

Rapid screening of banned pigments in food by liquid chromatography- tandem quadrupole time of flight mass spectrometry

Objective To establish a method for rapid screening of banned pigments in food by liquid chromatography-tandem quadrupole time of flight mass spectrometry (LC-QTOF-MS). Methods Information about 102 kinds of pigments was collected with regard to name, CAS number, neutral molecular formula and structure formula. Following that, the results were entered into the PCDL software. After the corresponding coloring solutions were prepared, the primary mass spectrum data of the solutions were obtained under the mode of MS. With the primary mass spectrum data confirmed, the target ions were given collision energy ranging from 10~60 eV under the mode of targeted MS/MS to acquire the secondary mass spectrum data. Results The primary and secondary mass spectrum spectral libraries were established for 102 kinds of pigments (including 16 kinds of edible pigments and 86 kinds of banned industrial dyes). Meanwhile, 8 groups of pigments with similar neutral molecular weights were separated. Conclusion The proposed method is rapid, accurate and sensitive, which is suitable for screening of the unknown banned pigments in food and rapid separation and quantitation of the pigments.