鲢鱼鱼鳞胶原蛋白抗氧化活性肽提纯及结构鉴定研究进展

为了利用鲢鱼鱼鳞生产胶原蛋白,采用生物酶解技术制备胶原蛋白抗氧化活性肽,可以提高水产品加工附加值且更好地保护环境。本文综述了鲢鱼鱼鳞胶原蛋白抗氧化活性肽的不同制备方式(包括固相化学合成法、液相化学合成法、酶水解法、DNA重组法等)和分离纯化方法(包括超滤法、离子交换色谱法、凝胶色谱法、反相高效液相色谱法等),并对其结构鉴定方式(包括质谱分析、连续流快原子轰击质谱、电喷雾离子化质谱、基质辅助激光解析等)做了阐述,以期为鲢鱼鱼鳞胶原蛋白抗氧化活性肽的进一步开发利用提供理论依据。

Research progress on purification and structure identification of antioxidant activity peptides of collagen in silver carp fish

In order to produce collagen protein using silver carp fish scale, biological enzymolysis technology is used for preparation of antioxidant activity peptides of collagen so as to improve the added value of the aquatic products processing and protect the environment better. In this paper, different preparation methods of antioxidant activity peptides of collagen of silver carp fish (including solid phase chemical synthesis, liquid phase chemical synthesis, enzymatic hydrolysis method, recombinant DNA method, etc.), separation and purification methods (including ultrafiltration method, ion exchange chromatography, gel chromatography, reversed-phase high performance liquid chromatography, etc.) and the structure identification methods (including mass spectrometry, continuous flow fast atom bombardment mass spectrometry, electrospray ionization mass spectrometry, matrix assisted laser resolution, etc.) were reviewed, so as to provide theoretical basis for further development and utilization of antioxidant activity peptides of collagen of silver carp fish.