| 转基因鲑鱼AquAdvantage品系特异性实时荧光聚合酶链式反应检测方法的建立 |
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| 引用本文： | 刘二龙,卢丽,吕英姿,蒋湘,李立霞,李嘉琪,杜雅萍,郑高彬. 转基因鲑鱼AquAdvantage品系特异性实时荧光聚合酶链式反应检测方法的建立[J]. 中国食品卫生杂志, 2017, 29(5): 576-580 |
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| 作者姓名： | 刘二龙 卢丽 吕英姿 蒋湘 李立霞 李嘉琪 杜雅萍 郑高彬 |
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| 作者单位： | 黄埔出入境检验检疫局,广东 广州 510730,广东出入境检验检疫局技术中心,广东 广州 510623,黄埔出入境检验检疫局,广东 广州 510730,黄埔出入境检验检疫局,广东 广州 510730,黄埔出入境检验检疫局,广东 广州 510730,黄埔出入境检验检疫局,广东 广州 510730,黄埔出入境检验检疫局,广东 广州 510730,黄埔出入境检验检疫局,广东 广州 510730 |
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| 基金项目： | 广东出入境检验检疫局科技项目(2017GDK41) |
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| 摘 要： | 目的实现转基因鲑鱼AquAdvantage的标识管理,建立其品系特异性实时荧光聚合酶链式反应(PCR)检测方法。方法针对转基因鲑鱼的品系特异性序列设计引物和TaqMan探针,建立转基因鲑鱼实时荧光PCR检测方法,并对该方法的特异性、灵敏度和重复性进行检测。结果建立的转基因鲑鱼实时荧光PCR方法特异性强,在600 000~60拷贝范围内呈良好的线性关系,其线性回归方程为y=-3.2194x+40.805,R~2=0.997,检测限为60拷贝,检测重复性良好。结论建立的品系特异性实时荧光PCR方法可应用于转基因鲑鱼AquAdvantage的鉴定。
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| 关 键 词： | 转基因鲑鱼 AquAdvantage 实时荧光聚合酶链式反应 品系特异性 检测 |
| 收稿时间： | 2017-05-05 |
Event-specific real-time polymerase chain reaction detection method of genetically modified AquAdvantage salmon |
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| LIU Er-long,LU Li,LYU Ying-zi,JIANG Xiang,LI Li-xi,LI Jia-qi,DU Ya-ping and ZHENG Gao-bin. Event-specific real-time polymerase chain reaction detection method of genetically modified AquAdvantage salmon[J]. Chinese Journal of Food Hygiene, 2017, 29(5): 576-580 |
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| Authors: | LIU Er-long LU Li LYU Ying-zi JIANG Xiang LI Li-xi LI Jia-qi DU Ya-ping ZHENG Gao-bin |
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| Affiliation: | Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangdong Guangzhou 510730,China,Guangdong Inspection and Quarantine Technology Center,Guangdong Guangzhou 510623,China,Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangdong Guangzhou 510730,China,Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangdong Guangzhou 510730,China,Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangdong Guangzhou 510730,China,Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangdong Guangzhou 510730,China,Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangdong Guangzhou 510730,China and Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangdong Guangzhou 510730,China |
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| Abstract: | Objective For implementation of labeling regulations, an event-specific real-time polymerase chain reaction(PCR) method for the detection of genetically modified AquAdvantage salmon was established in this study. Methods Primers and TaqMan probe were designed based on the event-specific sequence of AquAdvantage salmon. The specificity, sensitivity and repeatability of the developed method were examined, respectively. Results The specificity test of this method showed it was specific to AquAdvantage salmon. The 600 000-60 copies range showed a good linear relationship with Ct values, and its linear regression equation was y=-3.2194x+40.805(R2=0.997). The limit of quantification(LOQ) was 60 copies and the repeatability was good. Conclusion This event-specific real-time PCR method was suitable for the identification of genetically modified AquAdvantage salmon. |
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| Keywords: | Transgenic salmon AquAdvantage real-time polymerase chain reaction event-specific detection |
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