番茄红素对小鼠酒精性肝损伤的拮抗作用

目的探讨番茄红素对持续酒精摄入所致的酒精性肝损伤的拮抗作用。方法 100只健康成年SPF级昆明雄性小鼠随机分为5组:对照组、模型组和低、中、高剂量番茄红素组,每组20只。低、中、高剂量番茄红素组小鼠经口灌胃浓度分别为0.33、0.67和2.00 g/L的番茄红素玉米油混悬液,对照组与模型组灌胃等体积的玉米油,容量为0.2 ml/10 g,1次/d,连续30 d。第31 d始,给予上述试剂3 h后,对照组灌胃等量蒸馏水,其余4组灌胃56°白酒0.12 ml/10 g;1次/d,连续8 d。末次灌胃、禁食16 h后,测定血清中甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、蛋白质羰基,肝脏中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量,同时计算死亡率、累积存活时间。结果与对照组比较,模型组血清中TG、ALT、AST、蛋白质羰基和肝脏中MDA含量增加,差异有统计学意义(P0.01)。与模型组比较,中、高剂量番茄红素组小鼠死亡率降低,差异有统计学意义(P0.05),存活时间延长;同时,肝脏中SOD、GSH-Px含量均高于模型组,差异有统计学意义(P0.01),而血清中TG、ALT、AST、蛋白质羰基和肝脏中MDA含量降低,差异有统计学意义(P0.05,P0.01)。随着番茄红素灌胃剂量升高,小鼠血清中TG、ALT、AST、蛋白质羧基和肝脏中MDA含量呈下降趋势,而SOD、GSH-Px含量呈上升趋势。结论番茄红素对小鼠酒精性肝损伤具有一定的拮抗作用。

The antagonizing effect of lycopene on alcohol-induced liver injury in mice

To research the antagonizing effect of lycopene on alcohol-induced liver injury due to continuous ethanol intake in mice.Methods A total of 100 healthy adult of SPF male Kunming mice were randomly divided into 5 groups, including the control group, the model group, and the low, middle and high dose lycopene groups with different doses (0.33,0.67,2.00 g/L) of lycopene respectively by oral gavage at 0.2 ml/10 g once a day for 30 days, and the control group and model group were given corn oil. From the 31st day, 3 hours after administration, the control group was given distilled water, while the other 4 groups were given 56 degrees liquor by oral gavage at 0.12 ml/10 g once a day for 8 days. After the last administration, 5 groups were fasted for 16 h, then triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), protein carbonyl content in blood serum, and malonaldehyde (MDA), superoxide dismutase (SOD), glutathione perioxidase (GSH-Px) in live were tested, and mortality and cumulative survival time were calculated. Results Compared with the control group, the TG, ALT, AST, protein carbonyl and MDA level of the model group increased significantly (P<0.01). Compared with the model group, the mortality of middle and high dose groups was significantly reduced (P<0.05), and the cumulative survival time was extend. The SOD and GSH-Px levels were significantly higher (P<0.01), while the TG, ALT, AST, MDA and protein carbonyl content were obviously reduced (P<0.05, P<0.01). With the increasing lycopene doses, the TG, ALT, AST, MDA and protein carbonyl content were dropped, while the SOD and GSH-Px levels were on the rise.Conclusion Lycopene had a certain antagonizing effect on alcohol-induced liver injury in mice.