催化光度法测定三聚氰胺脱氨酶活性

建立一种测定三聚氰胺脱氨酶活性的催化光度法。利用Berthelot显色反应，通过测定单位时间内释放出的氨气量推算三聚氰胺脱氨酶的活性，并通过单因素试验和L9(34)正交试验优化测定条件。结果表明：最佳显色条件为水杨酸显色液加入量1.2mL、次氯酸钠溶液加入量0.3mL、显色反应时间40min、测定波长650nm；最佳酶促反应条件为三聚氰胺底物加入量0.2mL、粗酶液加入量60μL、酶促作用时间30min；在上述条件下，测得酶比活力为132.41U/L，批内批间变异系数范围分别为0.81%～1.19%和1.27%～1.69%。催化光度法对三聚氰胺脱氨酶酶活的测定，具有操作快速简便、灵敏度高、重复性好的优点。

Determination of Melamine Deaminase Activity by Catalytic Spectrophotometry

A method to determine melamine deaminase activity by catalytic spectrophotometry was developed based on Berthelot colour reaction.The melamine deaminase activity was calculated by the amount of ammonia released per minute.The reaction conditions for melamine deaminase were optimized by one-factor-at-a-time experiments combined with an orthogonal experiment design L9(34).Optimum coloration reaction condition was obtained when the reaction was allowed to proceed for 40 min in a system composed of 0.3 mL of so...