| 高效液相色谱-串联质谱法测食品中的赭曲霉毒素A |
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| 引用本文： | 史娜,路勇,吴颖,姜杰.高效液相色谱-串联质谱法测食品中的赭曲霉毒素A[J].食品科学,2011,32(18):260-263. |
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| 作者姓名： | 史娜 路勇 吴颖 姜杰 |
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| 作者单位： | 1.北京市食品安全监控中心
2.北京市产品质量监督检验所 |
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| 摘 要： | 研究建立高效液相色谱-串联质谱联用技术检测食品中赭曲霉毒素A的方法。根据不同样品，用甲醇-2%碳酸氢钠溶液(60:40，V/V)或甲醇-水(80:20，V/V)提取样品中的赭曲霉毒素A，经OchraTest亲和柱净化，以甲醇-5mmol/L(含0.1%甲酸)乙酸铵为流动相，采用正离子模式对赭曲霉毒素A进行检测。结果回收率在82.3%～98.5%之间，检出限为0.1μg/kg。该方法适用不同基质样品，准确性好、灵敏度高、抗干扰能力强，方法检出限可满足欧盟等最新限量要求。
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| 关 键 词： | 液相色谱- 串联质谱（LC-MS/MS） 赭曲霉毒素A 免疫亲和柱 多离子反应监测 |
| 收稿时间： | 2018-06-20 |
Analysis of Ochratoxin A in Foods by HPLC-MS/MS |
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| SHI Na,LU Yong,WU Ying,JIANG Jie.Analysis of Ochratoxin A in Foods by HPLC-MS/MS[J].Food Science,2011,32(18):260-263. |
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| Authors: | SHI Na LU Yong WU Ying JIANG Jie |
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| Affiliation: | (1. Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, China；
2. Beijing Products Quality Supervision and Inspection Institute, Beijing 100026, China) |
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| Abstract: | A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) method was proposed to determine ochratoxin A(OTA) in foods.Samples were extracted by methanol-2% sodium bicarbonate solution(60:40,V/V) or methanol-water(80:20,V/V),followed by clean-up on OchraTest affinity cartridge using methanol-5 mmol/L ammonium acetate solution in the presence of 0.1% formic acid as the mobile phase.OTA was detected in the in positive ion mode.The average spike recoveries for ochratoxin A in 4 food samples ... |
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| Keywords: | high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) ochratoxin A immuno-affinity column multiple reaction monitoring(MRM) |
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