| 牛IgG电化学纳米免疫传感器的研制 |
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| 引用本文： | 康晓斌,庞广昌,梁新义.牛IgG电化学纳米免疫传感器的研制[J].食品科学,2011,32(16):379-383. |
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| 作者姓名： | 康晓斌 庞广昌 梁新义 |
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| 作者单位： | 天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2006BAD04A00) |
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| 摘 要： | 研制特异性定量检测牛初乳制品及含牛IgG制品中牛IgG含量的电流型纳米免疫传感器。以成膜性及生物相容性良好的壳聚糖为媒介连接纳米金于玻碳电极，以比表面积大、吸附能力强、生物相容性好的纳米金固定兔抗牛IgG-HRP。通过循环伏安法及交流阻抗法表征电极组装各个过程的电化学特性，利用计时电流法测定PBS稀释的标准牛IgG。结果表明免疫前后稳定电流的变化率与标准牛IgG质量浓度的对数在0.1～10000ng/mL范围内线性相关，相关系数r=0.9976。该传感器稳定性及重现性良好，操作简单方便，成本较低，可用于含牛IgG制品中牛IgG含量的特异性定量检测。
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| 关 键 词： | 牛免疫球蛋白G（牛IgG） 免疫传感器 纳米金 计时电流法 |
Development of Nano-gold-modified Electrochemical Immunosensor for Bovine IgG |
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| KANG Xiao-bin,PANG Guang-chang,LIANG Xin-yi.Development of Nano-gold-modified Electrochemical Immunosensor for Bovine IgG[J].Food Science,2011,32(16):379-383. |
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| Authors: | KANG Xiao-bin PANG Guang-chang LIANG Xin-yi |
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| Affiliation: | (Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
Tianjin University of Commerce, Tianjin 300134, China) |
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| Abstract: | A current-type nano-immunosensor was developed for the specific determination of immunoglobulin G(IgG) content in colostrum products and bovine IgG-containing products.The chitosan with excellent film-forming capability and biocompatibility was used as the medium to connect nano-gold and glassy carbon electrode.Due to its large surface area,strong adsorption capacity and good biocompatibility,nano-gold was used to immobilize rabbit anti-bovine IgG-HRP.Electrochemical properties of electrode surface during e... |
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| Keywords: | bovine immunoglobulin G immunosensor nano-gold chronoamperometry |
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