高效液相色谱法测定芍药中齐墩果酸和熊果酸含量

目的：建立高效液相色谱法测定芍药不同器官中齐墩果酸和熊果酸含量的检测方法。方法：超声波乙醇提取样品中的齐墩果酸和熊果酸，色谱柱为Lichrospher C18柱(250mm×4.6mm，5μm)，流动相为甲醇-0.1%磷酸溶液(85:15，V/V)，柱温30℃，流速1mL/min，检测波长210nm。结果：齐墩果酸和熊果酸在25～100μg/mL范围内均呈良好的线性关系，相关系数均在0.998(n＝5)以上；日内差异系数和日间差异系数分别为1.27%～2.96%和1.33%～3.64%，OA和UA精确性分别为1.28%～3.52%和0.96%～2.88%，平均回收率分别为98.87%和98.70%。结论：该方法简单快速、结果准确，可为测定芍药不同组织中齐墩果酸和熊果酸含量提供一种高效快速的测定方法。

Analysis of Oleanolic and Ursolic Acids in Peony(Paeonia lactiflora Pall.) by HPLC

Objective: To establish a HPLC method to determine the contents of oleanolic acid (OA) and ursolic acid (UA) in different organs of Paeonia lactiflora Pall.. Method: Oleanolic and ursolic acids were extracted from samples with ethanol under the assistance of ultrasonic. The chromatographic separation was conducted on a Lichrospher C18 column (250 mm × 4.6 mm, 5μm) using a mobile phase made up of methanol and 0.1% phosphoric acid solution (85:15, V/V) at a flow rate of 1 mL/min. The column temperature and the detection wavelength were set as 30 ℃ and 210 nm, respectively. Results: The linearity of OA and UA was in the range of 25－100μg/mL with a correlation coefficient over 0.998 (n = 5). The overall intra-day and inter-day variation coefficients were 1.46%－2.84% and 1.33%－3.32% for OA and 1.27%－2.96% and 1.97%－3.64% for UA, respectively. The accuracy of determination of OA and UA was 1.28%－3.52% and 0.96%－2.88%, respectively. Conclusion: The method is convenient, rapid and accurate, and can thus be used to separate and quantify OA and UA in different organs of Paeonia lactiflora Pall..