| 南极磷虾酶解工艺优化及模型建立 |
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| 引用本文： | 吕传萍,李学英,杨宪时,郭全友. 南极磷虾酶解工艺优化及模型建立[J]. 食品科学, 2011, 32(18): 142-147. DOI: 10.7506/spkx1002-6630-201118030 |
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| 作者姓名： | 吕传萍 李学英 杨宪时 郭全友 |
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| 作者单位： | 1. 中国水产科学研究院东海水产研究所2.上海海洋大学食品学院 |
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| 基金项目： | 国家“863”计划项目(2011AA090801); 中央级公益性科研院所基本科研业务费专项(2009T05) |
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| 摘 要： | 以短肽得率(trichloroacetic acid-nitrogen soluble index，TCA-NSI)和水解度(degree of hydrolysis， DH)为指标，从7种常用酶中选出Alcalase酶作为酶解南极磷虾的最适酶。对Alcalase酶水解南极磷虾的酶用量、底物浓度、pH值、温度和时间5个因素进行单因素试验和正交旋转组合试验，建立TCA-NSI和DH与各因素的回归模型；在此基础上，结合实际生产确定Alcalase酶水解南极磷虾的最适工艺为温度50.7℃、pH8.01、加酶量3010U/g、时间239min，此时TCA-NSI值为73.02%，DH值为42.33%，短肽平均肽链长(peptide chain long，PCL)为2.36，平均相对分子质量为277.9。
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| 关 键 词： | 南极磷虾 Alcalase 短肽 水解度 |
Optimization and Modeling of Enzymatic Hydrolysis Process for Antarctic Krill |
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| LU Chuan-ping,LI Xue-ying,YANG Xian-shi,GUO Quan-you. Optimization and Modeling of Enzymatic Hydrolysis Process for Antarctic Krill[J]. Food Science, 2011, 32(18): 142-147. DOI: 10.7506/spkx1002-6630-201118030 |
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| Authors: | LU Chuan-ping LI Xue-ying YANG Xian-shi GUO Quan-you |
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| Affiliation: | (1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China；2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China) |
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| Abstract: | In the present work,alcalase was identified as the most suitable enzyme for enzymatic hydrolysis of Antarctic krill among 7 commonly used enzymes based on simultaneous consideration of chloroacetic acid-nitrogen soluble index(TCA-NSI) and degree of hydrolysis(DH).In order to optimize the hydrolysis of Antarctic krill by alcalase,the effects of the hydrolysis conditions enzyme dosage,substrate concentration,pH,temperature and hydrolysis time on TCA-NSI and DH were studied by one-factor-at-a-time and orthogon... |
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| Keywords: | Antarctic krill alcalase oligopeptide hydrolysis degree |
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