| 高效液相色谱法同时测定茶叶中8种儿茶素、3种嘌呤碱和没食子酸 |
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| 引用本文： | 李银花,李娟,龚雪,刘仲华. 高效液相色谱法同时测定茶叶中8种儿茶素、3种嘌呤碱和没食子酸[J]. 食品科学, 2011, 32(18): 214-217. DOI: 10.7506/spkx1002-6630-201118046 |
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| 作者姓名： | 李银花 李娟 龚雪 刘仲华 |
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| 作者单位： | 国家植物功能成分利用工程技术研究中心，湖南农业大学 茶学教育部重点实验室 |
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| 基金项目： | 湖南省重大科技专项(07FJ1005) |
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| 摘 要： | 建立高效液相色谱同时测定茶叶中8种儿茶素(EGC、DL-C、EC、EGCG、GCG、EGCG4O`Me、EGCG3O`Me、ECG)、3种生物碱(茶碱、可可碱、咖啡碱)和没食子酸的方法。色谱条件为Welchrom C18(250mm×4.6mm，5μm)色谱柱、磷酸缓冲液-乙腈溶液为流动相、梯度洗脱、波长278nm、流速1.0mL/min、柱温30℃。采用此方法，茶叶中各种成分都能达到基线分离的效果，线性关系良好，具有良好的稳定性和准确度。
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| 关 键 词： | 高效液相色谱 儿茶素 甲基化EGCG 嘌呤碱 没食子酸 |
Simultaneous Determination of Eight Catechins,Three Purine Alkaloids and Gallic Acid in Tea by High-performance Liquid Chromatography |
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| LI Yin-hua,LI Juan,GONG Xue,LIU Zhong-hua. Simultaneous Determination of Eight Catechins,Three Purine Alkaloids and Gallic Acid in Tea by High-performance Liquid Chromatography[J]. Food Science, 2011, 32(18): 214-217. DOI: 10.7506/spkx1002-6630-201118046 |
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| Authors: | LI Yin-hua LI Juan GONG Xue LIU Zhong-hua |
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| Affiliation: | (National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Key Laboratory ofTea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China) |
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| Abstract: | A method was developed to determine catechins, purine alkaloids and gallic acid in tea by HPLC-PDA. The optimum condition was achieved by separation on a Welchrom C18 column kept at 30 ℃ using phosphate buffer-acetonitrile as the mobile phase at a flow rate of 1.0 mL/min through gradient elution and detection at 278 nm. This method showed a good linearity for all 12 tested compounds. The results demonstrated high accuracy and stability of this method. |
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| Keywords: | high performance liquid chromatography(HPLC) catechin methylated EGCG purine alkaloid gallic acid |
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