| 复合酶法制备葛根多孔淀粉 |
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| 引用本文： | 江慧娟,黄赣辉.复合酶法制备葛根多孔淀粉[J].食品科学,2011,32(18):91-94. |
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| 作者姓名： | 江慧娟 黄赣辉 |
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| 作者单位： | 南昌大学 食品科学与技术国家重点实验室 |
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| 摘 要： | 使用α-淀粉酶与糖化酶复合制备葛根多孔淀粉。通过单因素试验，对多孔淀粉吸油率进行考察，研究其品质特性随加酶量、酶配比、pH值、酶解时间和酶解温度等变化的规律。并由正交试验得出最佳工艺条件，当加酶量0.6%、酶解时间12h、pH5.0、酶解温度50℃、酶质量比(糖化酶:α-淀粉酶)3:1时吸油率最高(60%)，且成孔效果良好。
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| 关 键 词： | 多孔淀粉 α-淀粉酶 糖化酶 酶解 吸油率 |
| 收稿时间： | 2018-06-20 |
Preparation of Porous Arrowroot Starch by Double Enzymatic Hydrolysis |
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| JIANG Hui-juan,HUANG Gan-hui.Preparation of Porous Arrowroot Starch by Double Enzymatic Hydrolysis[J].Food Science,2011,32(18):91-94. |
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| Authors: | JIANG Hui-juan HUANG Gan-hui |
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| Affiliation: | (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China) |
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| Abstract: | Porous arrowroot starch was prepared by simultaneous hydrolysis with α-amylase and glucoamylase. The effects of enzyme dosage, glucoamylase-to-α-amylase ratio, pH, hydrolysis time and hydrolysis temperature on the oil-absorbing capacity of porous arrowroot starch were investigated. The optimal hydrolysis conditions for preparing porous arrowroot starch were determined by orthogonal array design method to be 3:1 glucoamylase-to-α-amylase ratio, 0.6% total enzyme amount, pH 5.0, 12 h hydrolysis time and 50 ℃ hydrolysis temperature. Under these conditions, the maximum oil-absorbing rate of 3:1 and excellent pore formation were achieved. |
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| Keywords: | porous starch α-amylase glucoamylase hydrolysis oil-absorbing capacity |
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