FaptaSyme: A Strategy for Converting a Monomer/Oligomer‐Nonselective Aptameric Sensor into an Oligomer‐Selective One |
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Authors: | Baggio A. Evangelista Dr.Prof. Yoon‐Seong Kim Dr. Dmitry M. Kolpashchikov |
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Affiliation: | 1. Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA;2. Chemistry Department, University of Central Florida, Orlando, FL, USA;3. ITMO University, Laboratory of Solution Chemistry of Advanced Materials and Technologies, St. Petersburg, Russian Federation |
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Abstract: | Aptameric sensors can bind molecular targets and produce output signals, a phenomenon that is used in bioassays. In some cases, it is important to distinguish between monomeric and oligomeric forms of a target. Here, we propose a strategy to convert a monomer/oligomer‐nonselective sensor into an oligomer‐selective sensor. We designed an aptazyme that produced a high fluorescent output in the presence of oligomeric α‐synuclein (a molecular marker of Parkinson's disease) but not its monomeric form. The strategy is potentially useful in the design of point‐of‐care tests for the diagnosis of neurodegenerative diseases. |
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Keywords: | aptamers aptazymes biosensors deoxyribozymes split probes |
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