Magnetoelastic immunosensors: amplified mass immunosorbent assay for detection of Escherichia coli O157:H7 |
| |
| Authors: | Ruan Chuanmin Zeng Kefeng Varghese Oomman K Grimes Craig A |
| |
| Affiliation: | Department of Electrical Engineering, 217 Materials Research Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802, USA. |
| |
| Abstract: | A mass-sensitive magnetoelastic immunosensor for detection of Escherichia coli O157:H7 is described, based on immobilization of affinity-purified antibodies attached to the surface of a micrometer-scale magnetoelastic cantilever. Alkaline phosphatase is used as a labeled enzyme to the anti-E. coli O157:H7 antibody, amplifying the mass change associated with the antibody-antigen binding reaction by biocatalytic precipitation of 5-bromo-4-chloro-3-indolyl phosphate in a pH 10.0 PBS solution. The detection limit of the biosensor is 10(2) E. coli O157:H7 cells/mL. A linear change in the resonance frequency of the biosensor was found to E. coli O157:H7 concentrations ranging from 10(2) to 10(6) cells/mL. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
