麦胚蛋白降压肽的大孔树脂脱盐研究

采用大孔树脂对麦胚蛋白降压肽粗提物进行柱层析处理,以紫外吸收的峰面积来计算多肽的回收率,铬酸钾指示剂法测定氯的脱除率,灰分法测定总脱盐率,微量凯氏定氮法测定多肽含氮量及HPLC法测定多肽体外降压活性。结果表明:此脱盐方法简便可行。大孔树脂对麦胚蛋白降压肽粗提物的最大吸附能力为12.8mg/ml,氯的脱除率为92.31%,总脱盐率为90.14%,多肽回收率都大于85%,含氮率从5.23%提高到12.34%,体外降压活性由47.42%提高到70.40%。

Desalination of Antihypertensive Peptide Extracted from Wheat Germ Protein with Macroporous Resin Column Chromatography

The crude antihypertensive peptides extracted from wheat germ, were purified by chromatography on macroporousresin column. The recovery rate of antihypertensive peptides was defermined by ultraviolet absorbance spectrograde, whilechlorine content was determined by potassium chromate assay method. Total desalination rate calculated by ash content,nitrogen content assessed by KJedahl method and angiotensin converting enzyme inhibitor (ACEI) activity in vitro determinedby HPLC. Results: The desalination method was simple and feasible. The maximal disposal value of macroporous resin was 12.8mg/ml, chlorine remove rate 92.31%, the total desalination rate 90.14%, and recovery rate of antihypertensive peptides morethan 85%. In average, nitrogen content increased from 5.23% to 12.34%, and in vitro ACEI activity enhanced from 47.42% to70.40%.