牡丹花粉中4种重金属含量的测定与分析

目的 采用微波消解-原子吸收光谱法/原子荧光光谱法测定牡丹花粉中铅、镉、总汞、总砷含量，探明洛阳农博园地区牡丹花粉中4种重金属污染物状况。方法 选取6个主栽品种牡丹花粉样品，经微波消解后，采用原子吸收光谱法和原子荧光光谱法测定4种重金属污染物含量，并分析和评价测定结果。结果 铅、镉、总汞、总砷在一定浓度范围内线性关系良好，相关系数均大于0.998，方法检出限分别为 0.010、0.003、0.003、0.010 mg/kg，各元素的加标回收率在94.2%~109.4%之间。6个主栽品种牡丹花粉中均不同程度地检出了4种重金属污染物，方差分析结果显示，4种重金属元素含量的变异系数均大于10%，品种间差异均具有统计学意义。结论 本方法准确度高、精密度好。参考相关国家标准，6种牡丹花粉中重金属含量均符合要求，本研究为牡丹花粉资源的综合利用及牡丹花粉产品的开发提供数据支持。

Determination and analysis of 4 kinds of heavy metals content in peony pollen

Objective The content of lead, cadmium, total mercury and total arsenic in the pollen of peony was determined by microwave digestibility-atomic absorption spectrometry/atomic fluorescence spectrometry, in order to explore the status of 4 kinds of heavy metal pollutants in the pollen of peony in Luoyang agricultural expo park area. Methods The content of lead, cadmium, total mercury and total arsenic in the pollen of peony in 6 main cultivars was determined by microwave digestibility-atomic absorption spectrometry/atomic fluorescence spectrometry, and the determination results were analyzed and evaluated. Results The lead, cadmium, total mercury and total arsenic had a good linear relationship in a certain concentration range, the correlation coefficients were greater than 0.998, the limits of method detection were 0.010,0.003,0.003 and 0.010 mg/kg,The recoveries of each element were between 94.2%~109.4%. The 4 kinds of heavy metal pollutants were detected in the pollen of the 6 main cultivars, and the variance analysis results showed that the variation coefficients of the content of the 4 heavy metal elements were all greater than 10%, and the differences among different cultivars showed statistical significance. Conclusion The method has high accuracy and precision. According to the relevant national standard, the content of heavy metals in the pollen of 6 kinds of peony meets the requirements.This study provides data support for the comprehensive utilization of peony pollen resources and the development of peony pollen products.