| 响应面法优化丙酮酸发酵培养基 |
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| 引用本文： | 高年发,占桂荣. 响应面法优化丙酮酸发酵培养基[J]. 中国酿造, 2007, 0(5): 7-10 |
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| 作者姓名： | 高年发 占桂荣 |
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| 作者单位： | 天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300222 |
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| 摘 要： | 采用响应面法(RSM)对丙酮酸发酵培养基成分葡萄糖、硫酸铵、蛋白胨进行优化,采用多元二次回归方程拟合3种因素与丙酮酸含量间的函数关系,并得到了最佳条件。在优化培养条件下,发酵液中丙酮酸的浓度由35.4g/L提高到41.57g/L,在5L罐的最佳浓度下丙酮酸产量71.23g/L比原产量65.76g/L提高8.3%。
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| 关 键 词： | 丙酮酸 响应面分析 岭脊分析 |
| 文章编号： | 2054-0571(2007)05-0007-04 |
| 修稿时间： | 2006-12-14 |
Optimization of pyruvic acid fermentatiom medium by response surface method |
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| GAO Nian-fa,ZHAN Gui-rong. Optimization of pyruvic acid fermentatiom medium by response surface method[J]. China Brewing, 2007, 0(5): 7-10 |
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| Authors: | GAO Nian-fa ZHAN Gui-rong |
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| Abstract: | The composition of fermentation medium for the production of pyruvic acid by T.glabrata was optimized by response surface method(RSM).The functional relationship between the content of pyruvic acid and three factors,including maltose,glucose and tryptone,was fit using multi-power regression function method.Under optimized cultivation conditions,the concentration of pyruvic acid in broth increased from 35.4 g/L to 41.57 g/L,and the production of pyruvic acid was 71.23 g/L in 5 L fermentor,which was 8.3 % higher than the production of 65.76 g/L before optimization. |
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| Keywords: | pyruvic acid response surface analysis ridge analysis |
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