Simultaneous determination of vitamins A and E in feeds and foods by reversed phase high-pressure liquid chromatography

A simple high-pressure liquid chromatographic method for the simultaneous determination of vitamins A and E in animal feeds and foods has been developed and evaluated. After saponification and extraction the sample was run on a reversed-phase column with water-methanol as the mobile phase. The detector was an u.v.-lamp at 280 nm. The method has been tested on different types of feeds and foods containing between 2-30 μg vitamin A g^?1 of sample and 5-300 μg vitamin E g^?1 of sample. The recovery of added vitamins was 86 % for retinylacetate and 91 % for α-tocopheryl-acetate with a standard deviation of 3 % for both. Results of the proposed procedure agreed well with those obtained by other methods.