不同种植方式下烤烟根系差异表达蛋白质分析

通过根系差异蛋白质组学探讨了种植方式（复种连作、复种轮作）对烤烟生长的影响。结果表明，共有15个蛋白质表达丰度发生变化，用LC-MS/MS鉴定了14个差异蛋白质点，经生物信息学分析，11个蛋白质的功能有注释，其中3个属于与香气形成有关的蛋白质，包括转酮醇酶、单脱氢抗坏血还原酶和O-甲基转移酶，它们在复种轮作条件下均上调表达，此可能有助于烟叶品质提高，而复种连作处理下调表达，可能不利于品质提高。还鉴定到涉及能量、抗性、物质运输、核酸等功能的蛋白：磷酸甘油酸酯激酶、线粒体内膜移位酶亚基Tim13、NBS-LRR、DnaJ、细胞内囊泡运输蛋白Sly1、核糖体蛋白质，在复种轮作下也上调表达，此可能促进烤烟的生长，有利于提高烟草产量和品质且经济效益较好。而在复种连作处理下调表达，进而可能使得烟草生长变弱、产量降低、品质变差。

Analysis of Root Differential Expression Proteins of Nicotiana Tabacum underTwo Planting Systems

Root differential proteomics was employed in this study to explore the impact of different planting patterns （multiplecropping rotation （MR） and consecutive monoculture multiple cropping （MC） modes） on growth of flue-cured tobacco （Nicotinatobaccum L）. The results showed that a total of 15 protein spots had significant expression difference; 14 protein spots wereidentified by LC-MS/MS analysis and database searching. Among which, the function of 11 proteins were identified, three proteinswere related to the aroma formation, i.e. transketolase, monodehydroascorbate reductase（MDHA）, O-methyltransferase-like proteinwere up-regulated under MR, it was helpful probably to enhance the tobacco quality. While under the MC treatment, they weredown-regulated, and the tobacco quality could be worse. Phosphoglycerate kinase, mitochondrial import inner membrane translocasesubunit Tim13, NBS-LRR（nucleotide binding site plus leucine-rich repeat）, DnaJ protein, Vesicle trafficking protein Sly1, Ribosomalprotein-like protein were involved in energy metabolism, plant antioxidation reaction, translocation of solute, metabolism of nucleicacid, respectively. They were very important for the growth development of tobacco and all up-regulated under MR treatments,probably increasing the yield and improving the quality of tobacco, and gaining better economic benefit. They were alldown-regulated under MC, which may cause weak growth, low yield and poor quality of tobacco.