水通道蛋白4在脂多糖致大鼠感染性脑水肿模型中的表达变化

目的:观察脂多糖(LPS)致大鼠感染性脑水肿后水通道蛋白4的表达情况.探讨其在脑水肿形成发展中的作用.方法:84只雄性SD大鼠,随机分为3组:空白对照组(C组,n=12):生理盐水对照组(S组,n=12):水肿组(L组,n=60).水肿组又按注射脂多精后6h、12h、24h、48h、72h分为5个亚组(n=12).向颈...

Change of apuaporin4 in rats during brain edema induced by Hpid polysaccharide

Objective： To observe the expression of aquaporin - 4 （ AQP4 ） in rats with brain edema induced by lipid polysaccharide （ LPS ） as well as to expl6re the role of AQP4 in the formation and development of brain edema. Methods Eighty four SD male rats were randomly divided into 3 groups： control group （group C, n = 12）, saline group （ group S, n = 12）, LPS group （ group L, n = 60）. Group L was devided into 5 subgroups according to different time points after injecting LPS： 6h, 12h, 24h,48h, 72h （ each subgroup,n = 12）. Cerebral edema was induced by carotid injection with LPS （ 150pg/150ttL）. Dry - wet weight, evan blue, RT- PCR and immunohistechemistry methods were used respectively to measure the brain tissue water content, the integrity of BBB , the expression of AQP4 mRNA and AQP4 protein. Results： Compared with group C and group S, the brain tissue water content , EB content , AQP4 mRNA andprotein were significantly increased （P〈0.05） in group L,except subgroup 72h. They were increased at 6h after LPS injection,with a peak at 24h. No significant difference was found between group C and group S（P〉 0.05）. Conclusion： The results suggest that the expression of AQP4 is up regulated in brain edema induced by LPS, and it has positive relationship with the integrity of BBB and the brain tissue water content. It shows AQP4 may participat in the formation and development of infections cerebral edema.