| 低强度超声对鼻咽癌细胞线粒体膜电位的影响 |
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| 引用本文： | 王萍,夏新蜀,王听娜,徐静,向君彦,江园,许川山. 低强度超声对鼻咽癌细胞线粒体膜电位的影响[J]. 激光杂志, 2011, 0(1): 74-74 |
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| 作者姓名： | 王萍 夏新蜀 王听娜 徐静 向君彦 江园 许川山 |
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| 作者单位： | [1]川北医学院附属医院超声科,四川省南充637000 [2]重庆医科大学第一附属医院康复医学科,重庆400016 [3]重庆医科大学第二附属医院,重庆400010 |
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| 基金项目： | 本课题受国家高技术研究发展规划项目（863计划,2006A02ZAF0）和国家自然科学基金30973168）资助. |
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| 摘 要： | 目的:观察低强度超声对鼻咽癌细胞线粒体膜电位的影响.方法:以低强度超声(频率1.7 MHz,剂量1.35W/cm2)辐照鼻咽癌细胞,采用Rhodamine123染色送CLSM下检测鼻咽癌细胞线粒体膜电位的变化.结果:超声波能够量著损伤鼻咽癌CNE2细胞线粒体,辐照后孵育4h,CLSM 下光吸收值与对照组相比显著降低,结...
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| 关 键 词： | 低强度超声 线粒体膜电忙 鼻咽癌细胞 |
Effect of low- intensity ultrasound on mitochondrial membrane potential of Nasopharyngeal carcinonm cells |
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| Affiliation: | WANG PingI ,XIA Xin - shu(1 Department of Ultrasound, The Atfiliated Hospital of North Siehuan Medical College, Nanehong 637000, China; 2 Department of Rehabilitation Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016,China; 3 Department of Sonodynmnie therapy, The second Afllhated Hospital, Chongqing Medical University,Chongqing,400010, China ) |
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| Abstract: | Objective ： To investigate the effect of Low - intensity ultmsotmd on mitochondrial membrane potential of nasopharyngeal carcinoma cells. Methods： The nasopharyngeal carcinoma cells （CNE2 cells） were exposed to ultrasound with the frequency of 1.7MHz and the intensity of 1.35W/cm2）. After ultrasonic exposure, the mitochondrial membrane potential of nasopharyngeal carcinoma cells was observed by using eonfocal laser scanning microscopy （ CLSM ） with rhodamine 123 staining. Results： The mean fluorescent intensity of rhodamine 123 in the cells significantly decreased at 4h after ultrasound exposure as compared to control group. Conclusion： Low- intensity ultrasound could significantly induce the decrease of the mitochondrial membrane potential of nasopharyngeal carcinoma cells. |
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| Keywords: | low intensity ultrasound mitechondfial membrane potential nasopharyngeal carcinoma cells |
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