Hedgehog通路对乳腺癌细胞MDA—MB-231c—jun、uPA表达的影响和机制

目的：研究Hedgehog信号转导通路对人乳腺癌细胞MDA—MB-231c—jun、uPA表达的影响和作用机制，认识其在侵袭转移中的意义。方法：应用Hedgehog信号通路抑制剂Cyclopamine作用人乳腺癌细胞MDA—MB-231，Transwell小室进行侵袭迁移实验；Western blotting检测P—c...

The effect of hedgehog signaling pathway on c - jun, uPA expression of breast cancer cells MDA- MB - 231

Objective： To explore the effect and the possible mechanisms of Hedgehog singaling pathway on expression of c - jun, uPA in MDA - MB - 231 cells. Methods： Invasion and metastasis of MDA- MB- 231 cells were evaluated with transwell chamber.The expression of P- c- jun and uPA were detected by Western blot. The mRNA levels of Gli 1, c - jun and uPA were measured by reverse transcriplion - poly - merage chain reaction （ RT - PCR ）. Results ： When MDA - MB - 231 cells were treated with Cyclopamine, invasion and metastasis were depressed. The expression level of P- c- Jun and uPA were decreased. The mRNA levels of Ghl, c-jun and uPA were also reduced. Conclusion： invasion, metastasis and uPA level can be induced by activeled Hedgehog singnaling nathwav with activation of c - iun.