中间苍白杆菌对含蜡原油除蜡降粘分析

蜡质不仅使含蜡原油黏度升高，而且会析出积聚在管壁上降低管输效率。从原油污染淤泥中分离出一株产表面活性剂的烃降解菌F-1，经16S rDNA鉴定为中间苍白杆菌。与原油作用7 d 后，能将蜡含蜡量为15.2%的高含蜡原油处理为蜡含蜡量为9.1%的含蜡原油，原油蜡质量分数降低40.1%，原油黏度降低21%以上，细胞疏水性达28.1%。通过傅里叶红外光谱鉴定该菌产生的生物表面活性剂为脂肽类。添加该菌上清液到液体石蜡 可形成91.49 mm的排油圈，能够显著降低培养基表面张力，对液体石蜡乳化系数达到65%。

Analysis on Wax Removal and Viscosity Reduction of Waxy Crude Oil With Ochrobactrum intermedium

Waxy not only increases the viscosity of waxy crude oil, but also precipitates and accumulates on the pipe wall, reducing the efficiency of pipe transportation. A strain of surfactant producing hydrocarbon degrading bacteria F-1 was isolated from crude oil contaminated sludge and identified as Ochrobactrum intermedium by 16S rDNA. After the interaction with crude oil for 7 days, the high-wax crude oil with a wax content of 15.2% could be treated with a wax content of 9.1%, the crude oil wax mass fraction decreased by 40.1%, the crude oil viscosity decreased by more than 21%, and the cell surface hydrophobicity reached 28.1%. The biosurfactant produced by the bacteria was identified as lipopeptide by Fourier infrared spectroscopy. It can form a 91.49mm oil drainage ring, which can significantly reduce the surface tension of the medium and reach 65% emulsification coefficient of the liquid paraffin.