The use of nonhomologous scatchard analysis in the evaluation of ligand-protein interactions |
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Authors: | EJ van Zoelen RH Kramer HT van Moerkerk JH Veerkamp |
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Affiliation: | Department of Cell Biology, University of Nijmegen, The Netherlands. |
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Abstract: | Scatchard plots are widely used for the graphical presentation of receptor-ligand binding data. When a combination of labelled and unlabelled ligand molecules is used in a binding assay, equations for Scatchard plots are readily available if the labelled and unlabelled ligands have similar binding affinities. In this article, Everardus van Zoelen, Roel Kramer, Herman van Moerkerk and Jacques Veerkamp present mathematical equations to obtain the binding characteristics of an unlabelled ligand in a Scatchard plot, which has a dissociation equilibrium constant different from that of the labelled ligand used. |
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