Enhanced γ-CGTase Production by Bacillus Macorous with Membrane Active Substances |
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| Authors: | Feng Wang Guo-Cheng Du Yin Li Jian Chen |
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| Affiliation: |
a Key Laboratory of Industrial Biotechnology, Ministry of Education,
b Laboratory of Environmental Biotechnology, School of Biotechnology, Southern Yangtze University, China |
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| Abstract: | Some selected surfactants and alcohols were examined for their effects on γ-cyclodextrin glucanotransferase (γ-CGTase) production by Bacillus macorous WSH02-06. Both Tween 60 and n-Propyl alcohol had the ability to increase γ-CGTase yield about 1.7-fold. The investigation of fatty acid composition demonstrated that the addition of Tween 60 greatly increased the ratio of unsaturated to saturated fatty acids in cell membrane lipids, while n-propyl alcohol interfered with the proportion among unsaturated fatty acids. Study of the γ-CGTase fermentation in 5-L fermentor revealed that the addition of n-propyl alcohol improved the specific production rate and the yield of γ-CGTase. |
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| Keywords: | Bacillus macorous γ-CGTase membrane active substances n-propyl alcohol fatty acids membrane composition |
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