| 发酵液中乳酸的盐析萃取 |
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| 引用本文： | 魏搏超,宋志远,孙亚琴,修志龙. 发酵液中乳酸的盐析萃取[J]. 过程工程学报, 2012, 12(1): 44-48 |
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| 作者姓名： | 魏搏超 宋志远 孙亚琴 修志龙 |
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| 作者单位： | 大连理工大学生命科学与技术学院大连理工大学生命科学与技术学院大连理工大学环境与生命学院生物科学与工程系大连理工大学生命科学与技术学院 |
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| 摘 要： | 研究了一种利用盐析萃取法分离发酵液中乳酸的新方法. 通过系统考察乳酸在不同盐析萃取体系中的分配规律，发现K2HPO4-甲醇和K2HPO4-乙醇体系适合分离发酵液中的乳酸. 发酵液中乳酸浓度为167 g/L时，采用25%(w) K2HPO4-26%(w)甲醇盐析萃取体系，乳酸的分配系数和回收率分别为4.01和86.0%；采用14%(w) K2HPO4-30%(w)乙醇盐析萃取体系，乳酸的分配系数和回收率分别为3.23和90.6%. 此时上相中残余葡萄糖、菌体和可溶性蛋白的去除率分别达67.3%, 100%和85.9%.
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| 关 键 词： | 乳酸 盐析萃取 发酵液 |
| 收稿时间： | 2011-07-22 |
| 修稿时间： | 2012-01-10 |
Salting-out Extraction of Lactic Acid from Fermentation Broth |
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| WEI Bo-chao , SONG Zhi-yuan , SUN Ya-qin , XIU Zhi-long. Salting-out Extraction of Lactic Acid from Fermentation Broth[J]. Chinese Journal of Process Engineering, 2012, 12(1): 44-48 |
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| Authors: | WEI Bo-chao SONG Zhi-yuan SUN Ya-qin XIU Zhi-long |
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| Affiliation: | School of Life Science and Biotechnology, Dalian University of TechnologySchool of Life Science and Biotechnology, Dalian University of TechnologyDepartment of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of TechnologySchool of Life Science and Biotechnology, Dalian University of Technology |
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| Abstract: | Salting-out extraction was used for recovery of lactic acid from fermentation broth.The different hydrophilic organic solvents-inorganic salts systems were investigated for their extraction ability.The results showed that the K2HPO4-methanol and K2HPO4-ethanol systems appeared to be favorable.The partition coefficient and recovery rate of lactic acid reached up to 4.01 and 86.0% for the system of 25%(ω) K2HPO4-26%(ω) methanol,when the concentration of lactic acid was 167 g/L in the broth.For the system of 14%(ω) K2HPO4-30%(ω) ethanol,the partition coefficient and recovery rate of lactic acid reached up to 3.23 and 90.6%.The removal rate of glucose,cells and proteins reached 67.3%,100% and 85.9%,respectively. |
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| Keywords: | lactic acid salting-out extraction fermentation broth |
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