| 同时分离与测定鸡血中的五种喹诺酮药物的高效液相色谱法研究 |
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| 引用本文： | 刘平,李安运,陈星斌,陈金清,杨功俊. 同时分离与测定鸡血中的五种喹诺酮药物的高效液相色谱法研究[J]. 广东化工, 2011, 38(5): 211-212 |
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| 作者姓名： | 刘平 李安运 陈星斌 陈金清 杨功俊 |
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| 作者单位： | 1. 国家钨与稀土产品质量监督检验中心,江西,赣州,341000
2. 扬州大学,化学化工学院,江苏,扬州,225002 |
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| 摘 要： | 建立同时分离和测定五种喹诺酮类药物的高效液相色谱分析方法。本实验采用VP-ODS色谱柱,以甲醇-磷酸二氢钠缓冲溶液为流动相。五种喹诺酮类药物测定线性范围0.05～50.0μg/mL,最低检测限为0.003～0.005μg/mL。本方法简便、快速、灵敏度高,并运用于鸡体内的测定。
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| 关 键 词： | 反相高效液相色谱 鸡血浆 氟喹诺酮 |
The Simultaneous Determination of Five Quinolones in Chicken Plasma Sample Using Reversed-Phase High Performance Liquid Chromatography |
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| Liu Ping,Li Anyun,Chen Xingbin,Chen Jinqing,Yang Gongjun. The Simultaneous Determination of Five Quinolones in Chicken Plasma Sample Using Reversed-Phase High Performance Liquid Chromatography[J]. Guangdong Chemical Industry, 2011, 38(5): 211-212 |
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| Authors: | Liu Ping Li Anyun Chen Xingbin Chen Jinqing Yang Gongjun |
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| Affiliation: | 1.China Quality Supervision and Inspection Center of Tungsten and Rare-earth Products,Ganzhou 341000;2.College of Chemistry and Chemical Engineering of Yangzhou University,Yangzhou 225002,China) |
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| Abstract: | High performance liquid chromatography(HPLC) was developed for the simultaneous determination of five quinolones in chicken plasma.The experiments of HPLC were carried out on a VP-ODS column using phosphate buffer solution: methanol.The calibration curves were linear in the concentration range from 0.05～50.0 μg/mL.The detection limit of five QNs was 0.003～0.005 μg/mL.The method was validated for accuracy and precision,and it was successfully applied to plasma samples from chicken that had been orally administered. |
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| Keywords: | reversed-phase high-performance liquid chromatograph chicken plasma quinolones |
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