Comparison of Size Characterization of Barley Starch Granules Determined by Electron and Optical Microscopy,Low Angle Laser Light Scattering and Gravitational Field-Flow Fractionation |
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Authors: | J Chmelík A Krumlová M Budinská T Kruml V Psota I Bohac̆enko P Mazal H Vydrová |
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Affiliation: | 1. Institute of Analytical Chemistry, Vever̆í 97, 611 42 Brno, Czech Republic;2. Institute of Physics of Materials, z̆iz̆kova 22, 616 62 Brno, Czech Republic;3. Research Institute of Brewing and Malting, Mostecká 7, 614 00 Brno, Czech Republic;4. Food Research Institute Prague, Radiová 7, 102 37 Prague, Czech Republic;5. Mendel University of Agriculture and Forestry, Lesnická 3, 613 00 Brno, Czech Republic;6. Institute of Chemical Technology, 160 00 Prague, Czech Republic |
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Abstract: | Several methods were used for the characterization of starch granules isolated from barley kernels. A procedure based on a combination of alkaline digestion, toluene treatment and filtration over sieves with pore diameters of 70 and 40 μm was used for isolation and purification of starch granules from kernels. The released starch granules were characterized by various methods: scanning electron microscopy (SEM), image analysis of optical microscopy data (IAOM), low angle laser light scattering (LALLS), and gravitational field-flow fractionation (GFFF). All methods showed the bimodal size distribution of the isolated starch granules, however, they differed in the ratio of large and small starch granules. LALLS and GFFF were also used for determination of the ratio of large and small starch granules (ratio A/B) isolated from two malting barley cultivars Kompakt and Akcent. Both techniques determined the higher ratio A/B for the cultivar Akcent. SEM was also used to examine the extent of digestion. The micrographs indicate that a significant proportion mainly of small granules are still embedded into residues of endosperm and a more extensive digestion must be performed to release all starch granules from barley kernels. |
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Keywords: | Key Words: Barley starch granules size determination electron microscopy optical microscopy image analysis low angle laser light scattering gravitational field-flow fractionation |
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