| 肉桂酸生物合成苯乙酮微生物的筛选及反应条件优化 |
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| 引用本文： | 马丽,刘雄民,暴玮,梁静娟.肉桂酸生物合成苯乙酮微生物的筛选及反应条件优化[J].精细化工,2008,25(8). |
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| 作者姓名： | 马丽 刘雄民 暴玮 梁静娟 |
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| 作者单位： | 1. 广西大学,化学化工学院,广西,南宁,530004
2. 广西大学,生命科学与技术学院,广西,南宁,530004 |
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| 基金项目： | 广西壮族自治医科学基金资助项目
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广西壮族自治区应用基础研究项目 |
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| 摘 要： | 以肉桂酸为碳源,从肉桂种植区土壤中富集筛选获得了一株能将肉桂酸高效、高选择性地转化为苯乙酮的菌株JX66,经鉴定,菌株JX66为越南伯克氏菌(Burkholderia vietnam iensis)。研究表明,在肉桂酸质量浓度为5 g/L,反应体系的初始pH=9.0,28~30℃,反应48 h,Burkholderia vietnam iensisJX66催化肉桂酸转化为苯乙酮的转化率为83.5%;反应产物为单一的苯乙酮。
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| 关 键 词： | 生物催化 肉桂酸 苯乙酮 越南伯克氏菌 生物工程 |
Screening of Strain for Acetophenone Production from Cinnamic Acid and Optimization of the Reaction Conditions |
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| MA Li,LIU Xiong-min,BAO Wei,LIANG Jing-juan.Screening of Strain for Acetophenone Production from Cinnamic Acid and Optimization of the Reaction Conditions[J].Fine Chemicals,2008,25(8). |
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| Authors: | MA Li LIU Xiong-min BAO Wei LIANG Jing-juan |
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| Abstract: | A strain JX66 was screened from the soil of Chinese cinnamon tree plantation by enrichment technique.Cinnamic acid(CMA) could be transformed to acetophenone(ACP) by it efficiently.The strain JX66 was identified as Burkholderia vietnamiensis.The appropriate conditions for the reaction were cinnamic acid 5 g/L,pH=9.0,28～30 ℃ and 48 h reaction time.The conversion rate of cinnamic acid was 83.5%.ACP was the only reaction product. |
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| Keywords: | biocatalysis cinnamic acid acetophenone Burkholderia vietnamiensis bioengineering |
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