| 反相高效液相色谱法测定女贞子中桦木醇含量 |
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| 引用本文： | 张秋丰,弓晓杰. 反相高效液相色谱法测定女贞子中桦木醇含量[J]. 大连工业大学学报, 2010, 29(1) |
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| 作者姓名： | 张秋丰 弓晓杰 |
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| 作者单位： | 1. 大连大学,环境与化学工程学院,辽宁,大连,116622
2. 大连大学,医学院,辽宁,大连,116622 |
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| 摘 要： | 采用反相高效液相色谱法测定女贞子中桦木醇含量,考察了不同溶剂提取方法对女贞子中桦木醇含量测定的影响。色谱柱,Supelcosil LC-PAH C18(250 mm×4.6 mm,5μm);流动相,V(乙腈)∶V(水)∶V(磷酸)=830∶170∶1;体积流量,1.0 mL/min;检测波长,210 nm;采用外标法测定桦木醇含量。结果表明,使用正丁醇和超声提取女贞子中桦木醇的含量较高。桦木醇在2~20μg与峰面积具有良好线性关系,平均回收率为98.87%,RSD为1.91%(n=5)。该方法简便、稳定、重现性好,可用于女贞子药材的质量控制。
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| 关 键 词： | 女贞子 桦木醇 反相高效液相色谱 |
Determination of betulin in Fructus ligustri lucidi by RP-HPLC |
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| ZHANG Qiu-feng,GONG Xiao-jie. Determination of betulin in Fructus ligustri lucidi by RP-HPLC[J]. Journal of Dalian Dalian Polytechnic University, 2010, 29(1) |
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| Authors: | ZHANG Qiu-feng GONG Xiao-jie |
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| Abstract: | The method with different solvents for exacting betulin from Fructus ligustri lucidi was investigated via HPLC on Supelcosil LC-PAH C18(250 mm×4.6 mm,5 μm),mobile phase [830 mL acetonitrile∶170 mL water∶1 mL H3PO4],the flow rate 1.0 mL/min,the detection wavelength at 210 nm.The content of Betulin was determined with external standard method.The yield of betulinic was higher with ultrasonics in 1-Butanol than that with others.The standard curves of Betulin showed good linearity over the range of 2-20 μg and the average recovery was 98.87% with RSD 1.91%(n=5).The method is simple,stable,accurate and reliable for the quality control of Fructus ligutri lucidi. |
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| Keywords: | Fructus ligutri lucidi betulin RP-HPLC |
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