| Electrochemical mechanism of rusticyanin （Rus.） isolated from A. ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode |
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| 引用本文： | 孙静,余润兰,苗雷,钟代立,刘杰,顾帼华.Electrochemical mechanism of rusticyanin （Rus.） isolated from A. ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode[J].中南工业大学学报(英文版),2011,18(5):1389-1394. |
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| 作者姓名： | 孙静 余润兰 苗雷 钟代立 刘杰 顾帼华 |
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| 作者单位： | Key Laboratory of Biometallurgy of Ministry of Education;School of Minerals Processing and Bioengineering;Central South University; |
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| 基金项目： | Project(2010CB630903) supported by the National Basic Research Program of China; Project(50621063) supported by the National Natural Science Foundation of China |
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| 摘 要： | Electrochimcal behaviors of rusticyanin (Rus.) isolated from Acidithiobacillus ferrooxidans were investigated through Rus.-ZnS-QDs/L-Cys/Au electrode.The cyclic voltammetric results indicate that rusticyanin immobilized on the surface of Rus.-ZnS-QDs/L-Cys/Au electrode can undergo a direct quasi-reversible electrochemical reaction.The immobilized rusticyanin is not denatured and still retains its activity in the temperature range of 19-43 °C.The reduction ability of the protein increases and its oxidation a...
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| 关 键 词： | 氧化亚铁硫杆菌 电化学反应 电极 分离 机制 测量 氧化能力 反应动力学 |
| 收稿时间： | 24 May 2010 |
Electrochemical mechanism of rusticyanin (Rus.) isolated from A. ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode |
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| Jing Sun, Run-lan Yu, Lei Miao, Dai-li Zhong, Jie Liu and Guo-hua Gu.Electrochemical mechanism of rusticyanin (Rus.) isolated from A. ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode[J].Journal of Central South University of Technology,2011,18(5):1389-1394. |
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| Authors: | Jing Sun Run-lan Yu Lei Miao Dai-li Zhong Jie Liu and Guo-hua Gu |
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| Affiliation: | (1) State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People’s Republic of China; |
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| Abstract: | Electrochimcal behaviors of rusticyanin (Rus.) isolated from Acidithiobacillus ferrooxidans were investigated through Rus.-ZnS-QDs/L-Cys/Au electrode. The cyclic voltammetric results indicate that rusticyanin immobilized
on the surface of Rus.-ZnS-QDs/L-Cys/Au electrode can undergo a direct quasi-reversible electrochemical reaction. The immobilized
rusticyanin is not denatured and still retains its activity in the temperature range of 19–43 °C. The reduction ability of
the protein increases and its oxidation ability becomes weak with the increase of pH from 6.0 to 7.8. Fe2+ ions in the solution can promote the electron transfer kinetics of the immobilized rusticyanin and make its peak potentials
(φ
p) markedly move negatively. |
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| Keywords: | rusticyanin acidithiobacillus ferrooxidans cyclic voltammetry electrochemistry |
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