| 光度法测定药品和食物中的微量VC |
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| 引用本文： | 刘宇奇,杨睿,杨泳.光度法测定药品和食物中的微量VC[J].昆明理工大学学报(理工版),2008,33(3):112-115. |
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| 作者姓名： | 刘宇奇 杨睿 杨泳 |
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| 作者单位： | 1. 昆明理工大学理学院,云南昆明,650093
2. 昆明医学院药理教研室,云南昆明,650031 |
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| 基金项目： | 昆明理工大学科研启动基金 |
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| 摘 要： | 采用一种简单、快速的方法测定VC,该方法基于在室温下,抗坏血酸能快速地将Fe3 还原成Fe2 ,Fe2 与2,2’-联吡啶反应生成红色配合物,配合物的最大吸收峰位于520 nm波长处,VC的质量浓度在0.088~7.0 mg/L范围内符合比尔定律,该方法用于食品和药片中VC含量的测定,结果的相对标准偏差小于1.5%,回收率在96.3%~105.0%之间.
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| 关 键 词： | 分光光度法 联毗啶 维生素C 含量测定 |
Determination of Vitamin C in Foods and Medical Tablet by Spectrophotometry |
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| LIU Yu-qi,YANG Rui,YANG Yong.Determination of Vitamin C in Foods and Medical Tablet by Spectrophotometry[J].Journal of Kunming University of Science and Technology(Natural Science Edition),2008,33(3):112-115. |
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| Authors: | LIU Yu-qi YANG Rui YANG Yong |
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| Abstract: | A simple and fast method is used for the determination of VC in this paper.This method is based on the fact that under room temperature,ascorbic acid reduces Fe(III) to Fe(II) quickly and the latter reacts with bipyridine(2,2'-hipy) to form a reddish colored complex with its absorption maximum at the wavelength of 520 nm.Beer's law is obeyed in the concentration range of 0.088-7.0 mg of VC per 1000mL of solution.The proposed method is then applied to the determination of foods and medical tablet.RSD's(n=6) is less than 1.5% with recoveries in the range of 96.3%-105.0%. |
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| Keywords: | spectrophotometry vitamin C bipyridine content determince |
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