猪附红细胞体ORF2蛋白的二级结构及其B细胞抗原表位的预测

目的预测猪附红细胞体亦称猪嗜血支原体(Mycoplasma suis,M.suis)]ORF2基因编码蛋白的二级结构及其B细胞抗原表位。方法应用生物信息学软件DNAstar的Editseq将M.suis中ORF2的基因核苷酸序列翻译成氨基酸序列,与GenBank中登录的氨基酸序列进行比对,通过Protean模块预测ORF2蛋白的二级结构及其亲水性区域、柔韧性区域、抗原指数、表面可及性等特性,并预测B细胞优势抗原表位。结果 ORF2蛋白具有规则的二级结构,多处于亲水性区域、柔韧性区域、表面可及性较大和抗原指数较高的区段,潜在的优势B细胞抗原表位为17²3、12123、121¹27、138127、138¹44、192144、192²00氨基酸区段。结论预测了ORF2蛋白二级结构和B细胞抗原表位,为M.suis表位疫苗的设计以及血清诊断试剂盒的研发奠定了理论基础。

Prediction of secondary structure and B-cell antigenic epitopes of Mycoplasma suis ORF2 protein

Objective To predict the secondary structure and B-cell antigenic epitopes of Mycoplasma suis ORF2.Methods The nucleotide sequence of ORF2 of M.suis was translated to amino acid sequence by using the Editseq program in DNAstar software,and compared with that reported in GenBank,based on which the secondary structure,hydrophilic region,flexible region,antigenic index,surface probability as well as prominent antigenic epitopes of B cells were predicted by Protean module.Results The ORF2 protein showed regular secondary structure,abundant hydrophilic and flexible regions,as well as the regions with large surface probability and the high antigenic index.The potential prominent antigenic epitopes of B cells were located in 17 ~ 23,121 ~ 127,138 ~ 144 and 192 ~ 200 aa.Conclusion The secondary structure and prominent antigenic epitopes of B cells in ORF2 of M.suis was predicted,which provided a theoretical basis for design of M.suis epitope vaccine and development of serological diagnostic kit.