| 骨形态发生蛋白9-2双表达定向诱导多潜能干细胞C3H10的成骨分化 |
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| 引用本文： | 陈聪,罗庆,田雯,迭小红,康权.骨形态发生蛋白9-2双表达定向诱导多潜能干细胞C3H10的成骨分化[J].粉末涂料与涂装,2013,26(1):32-35. |
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| 作者姓名： | 陈聪 罗庆 田雯 迭小红 康权 |
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| 作者单位： | 重庆医科大学附属儿童医院儿童发育疾病研究省部共建教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆,400014 |
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| 基金项目： | 国家自然科学基金(30973062);重庆市自然科学基金(CSTC2008BB5390,CSTC2009BB5408) |
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| 摘 要： | 目的探讨骨形态发生蛋白(Bone morphogenetic protein,BMP)9-2双表达定向诱导多潜能干细胞C3H10向成骨细胞分化的情况。方法将C3H10细胞分为4组:BMP9-2、BMP2、BMP9和GFP组,将4种重组腺病毒分别感染C3H10细胞,通过碱性磷酸酶(Alkaline phosphatase,ALP)染色、定量测定及钙茜素红染色,观察BMP9-2双表达对C3H10细胞成骨分化的定向诱导作用。结果 BMP9-2双表达能诱导C3H10细胞ALP的表达,其染色活性高于BMP2组1.6倍,BMP9组2.5倍,GFP组4倍;其BMP9-2双表达定量表达A520值在病毒感染后5、7、9 d均高于BMP2、BMP9和GFP组;BMP9-2双表达能诱导C3H10细胞钙盐的沉积,感染14 d后,钙茜素红染色可见钙化结节,其染色活性高于BMP2组1.4倍,BMP9组1.3倍,GFP组5.2倍。结论 BMP9-2双表达能定向诱导C3H10细胞成骨分化,其成骨活性强于单一成骨诱导因子BMP2和BMP9。
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| 关 键 词： | 骨形态发生蛋白质类 双表达 多潜能干细胞 C3H10细胞 成骨 重组腺病毒 |
Co-expression of bone morphogenetic protein 9-2 induces differentiation of multipotential stem cells C3H10 into osteoblasts |
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| CHEN Cong,LUO Qing,TIAN Wen,DIE Xiao-hong,KANG Quan.Co-expression of bone morphogenetic protein 9-2 induces differentiation of multipotential stem cells C3H10 into osteoblasts[J].Chinese Journal of Biologicals,2013,26(1):32-35. |
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| Authors: | CHEN Cong LUO Qing TIAN Wen DIE Xiao-hong KANG Quan |
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| Affiliation: | Ministry of Education Key Laboratory of Child Development and Disorders,Key Laboratory of Pediatrics in Chongqing,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders,Children’s Hospital of Chongqing Medical University,Chongqing 40014,China |
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| Abstract: | Objective To investigate the capacity of co-expression of bone morphogenetic protein 9-2(BMP9-2) in inducing the differentiation of multipotential stem cells C3H10 into osteoblasts.Methods C3H10 cells were divided into four groups,and infected with recombinant adenoviruses with BMP9-2,BMP2,BMP9 and GFP respectively.The induction of differentiation of C3H10 cells to osteogenesis by co-expression of BMP9-2 was observed by alkaline phosphatase(ALP) staining,alkaline phosphatase quantitation and calcium-alizarin red staining.Results The co-expression of BMP9-2 induced the expression of ALP in C3H10 cells.The positive rate of ALP in BMP9-2 group was 1.6 times higher than that in BMP2 group,2.5 times higher than that in BMP9 group,and 4 times higher than that in GFP group.The activities(A520) of ALP in BMP9-2 group 5,7 and 9 d after infection were higher than those in BMP2,BMP9 and GFP groups.The co-expression of BMP9-2 induced the sedimentation of calcium salt in C3H10 cells.Calcified tuberculum was observed in C3H10 cells 14 d after infection with BMP9-2 by calcium-alizarin red staining.The sedimentation level in BMP9-2 group was 1.4 times higher than that in BMP2 group,1.3 times higher than that in BMP2 group,and 5.2 times higher than that in GFP group.Conclusion The co-expression of BMP9-2 may induce the differentiation of C3H10 cells into osteoblasts,of which the ability in inducing osteogenesis is higher than those of BMP2 and BMP9. |
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| Keywords: | Bone morphogenetic proteins Co-expression Pluripotent stem cells C3H10 cells Osteogenesis Recombinant adenovirus |
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