| 超声微波协同萃取-HPLC测定五倍子中的没食子酸含量 |
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| 引用本文： | 李红然,付大友,李艳清,唐侄香,孙晓芳,张伶俐.超声微波协同萃取-HPLC测定五倍子中的没食子酸含量[J].化工时刊,2011,25(1):36-38,41. |
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| 作者姓名： | 李红然 付大友 李艳清 唐侄香 孙晓芳 张伶俐 |
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| 作者单位： | 四川理工学院,材料与化学工程学院,四川,自贡,643000 |
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| 摘 要： | 采用高效液相色谱法,以C18色谱柱为分析柱,乙腈-0.2%磷酸溶液(7∶ 93)为流动相,检测波长273 nm,柱温25℃,并利用超声微波协同萃取法对没食子酸的提取进行单因素的考察,建立超声微波协同萃取-HPLC法测定五倍子中没食子酸含量.没食子酸进样量在1～5 μg范围内峰面积与其浓度有良好的线性关系(r=0.999...
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| 关 键 词： | 五倍子 没食子酸 高效液相色谱(HPLC) 超声微波协同萃取 |
Determination of Gallic acid in Galla Chinensis of Herbal by Ultrasonic Wave and Microwave Synergic Extraction-HPLC |
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| Li Hongran,Fu Dayou,Li Yangqing,Tang Zhixiang,Sun Xiaofang,Zhang Lingli.Determination of Gallic acid in Galla Chinensis of Herbal by Ultrasonic Wave and Microwave Synergic Extraction-HPLC[J].Chemical Industry Times,2011,25(1):36-38,41. |
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| Authors: | Li Hongran Fu Dayou Li Yangqing Tang Zhixiang Sun Xiaofang Zhang Lingli |
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| Affiliation: | Li Hongran Fu Dayou Li Yangqing Tang Zhixiang Sun Xiaofang Zhang Lingli(Material and Chemical Engineering College,Sichuan University of Science and Engineering,Zigong 643000) |
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| Abstract: | The determination of gallic acid in galla chinensis by ultrasonic wave and microwave synergic extraction-HPLC was set up.The condition of HPLC was as follows: AQCl8(150 mm×4.6 mm,5 μm)column,acetonitrile-0.2%phosphoric aid(7∶ 93) as mobile phase,with a detection wavelength of 273 nm and the temperature to 25℃.The effects of factors on the extraction of gallic acid was examined through single factor experiment.Results showed that the linear relationship was good in the range of 1~5 μg for gallic acid.The recovery rate was 95.62% with RSD=1.58%(n=5).The suitable conditions was microwave power of 230W,ultrasonic wave power of 50W,the time of 810s and the liquid-solid ratio of 1/30.The method was convenient,rapid and accurate,and can be used for the extraction and qualitative and quantitative analysis of gallic acid. |
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| Keywords: | galla chinensis gallic acid HPLC ultrasonic wave and microwave synergic extraction |
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