| 逆境诱导烟草悬浮细胞内处理小体的形成 |
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| 引用本文： | 莫蓓莘,叶浩,欧忠华,刘丽,黄彪,朱双琳. 逆境诱导烟草悬浮细胞内处理小体的形成[J]. 深圳大学学报(理工版), 2012, 29(1): 80-84 |
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| 作者姓名： | 莫蓓莘 叶浩 欧忠华 刘丽 黄彪 朱双琳 |
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| 作者单位： | 1. 深圳大学生命科学学院,深圳,518060
2. 深圳大学生命科学学院,深圳518060;麦克马斯特大学健康科学系,加拿大哈密尔顿L8S4L8 |
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| 基金项目： | 国家自然科学基金资助项目，广东省自然科学基金自由申请项目基金资助 |
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| 摘 要： | 指出处理小体( processing body,P-body)在真核细胞基因表达调节过程中,特别是在逆境条件下,能为mRNA在细胞质内转运提供储存和降解场所.研究通过农杆菌转化法成功将含有处理小体荧光标记物-脱帽酶1( decapping enzyme,DCP1)与青色荧光蛋白(cyan fluorescent protein,CFP)融合蛋白基因的植物表达载体转入烟草悬浮细胞.利用激光共聚焦显微镜观察到在高温和盐胁迫下,处理小体的数量和体积都有增加.通过蔗糖密度梯度离心分析逆境条件下多核糖体的变化,发现在高温和盐胁迫下,多核糖体解体,呈现翻译抑制特征.
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| 关 键 词： | 植物细胞学 处理小体 多核糖体 脱帽酶1 逆境胁迫 信使核糖核酸 烟草悬浮细胞-BY-2 青色荧光蛋白 |
Stress-induced processing body formation in tobacco suspension cells |
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| MO Bei-xin , YE Hao , OU Zhong-hua , LIU Li , HUANG Biao , ZHU Shuang-lin. Stress-induced processing body formation in tobacco suspension cells[J]. Journal of Shenzhen University(Science &engineering), 2012, 29(1): 80-84 |
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| Authors: | MO Bei-xin YE Hao OU Zhong-hua LIU Li HUANG Biao ZHU Shuang-lin |
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| Affiliation: | 1,2 1) College of Life Science,Shenzhen University,Shenzhen 518060,P.R.China 2) Department of Health Science,McMaster University,Hamilton L8S4L8,Canada |
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| Abstract: | Processing bodies(P-body) serve as key functions in regulating gene expression in eukaryotic cells,and especially under stress conditions,serve as stress-induced mRNA relocalization sites for mRNA degradation or storage.We successfully transformed P-body fluorescent marker(DCP1 and CFP fusion protein) containing plasmid into suspension BY-2 cells.Confocal observation found that P-body increased in abundance and size under heat and salt stress conditions.Sucrose gradient ultra-centrifugation analysis demonstrated that both heat and salt stress conditions induced polysome run-off.This is characteristic of inhibition of translation initiation. |
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| Keywords: | plant cytology processing body polysome decapping enzyme(DCP1) stress condition mRNA tobacco suspension cells-BY-2 cyan fluorescent protein(CFP) |
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