| BSA修饰Fe_3O_4纳米颗粒用于苦瓜提取液中活性成分的筛选 |
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| 引用本文： | 张银堂,陈芳,杨政文,孙清臣,徐茂田.BSA修饰Fe_3O_4纳米颗粒用于苦瓜提取液中活性成分的筛选[J].应用化工,2010,39(9). |
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| 作者姓名： | 张银堂 陈芳 杨政文 孙清臣 徐茂田 |
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| 作者单位： | 商丘师范学院,化学系,纳米生物分析化学河南省高校重点实验室培育基地,河南,商丘,476000 |
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| 基金项目： | 国家自然科学基金项目，河南省教育厅自然科学基础研究计划项目，商丘师范学院青年科学基金项目 |
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| 摘 要： | 用一步法直接合成了表面氨基化修饰的Fe3O4纳米颗粒(NH2-MION),通过双功能团试剂戊二醛将牛血清白蛋白(BSA)固定在NH2-MION表面,将BSA-MION用于苦瓜提取液中活性成分的筛选,利用气相色谱-质谱联用技术鉴定出了3种与BSA作用较强的活性成分。
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| 关 键 词： | Fe3O4纳米颗粒 牛血清白蛋白 苦瓜 活性成分 筛选 |
Active ingredient screening in momordica charantia using BSA immobilized magnetic iron oxide nanoparticles |
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| ZHANG Yin-tang,CHEN Fang,YANG Zheng-wen,SUN Qing-chen,XU Mao-tian.Active ingredient screening in momordica charantia using BSA immobilized magnetic iron oxide nanoparticles[J].Applied chemical industry,2010,39(9). |
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| Authors: | ZHANG Yin-tang CHEN Fang YANG Zheng-wen SUN Qing-chen XU Mao-tian |
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| Abstract: | Amine coated magnetic iron oxide nanoparticles(NH2-MION) were prepared by one-step process.Bovine serum albumin(BSA) was immobilized on the surface of NH2-MION via double functional group reagent glutaraldehyde.Active ingredient screening in momordica charantia was implemented via BSA coated magnetic iron oxide nanoparticles(BSA-MION).Three active ingredients were identified by gas chromatography-mass spectrometry(GC-MS). |
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| Keywords: | magnetic iron oxide nanoparticles bovine serum albumin momordica charantia active ingredient screening |
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