Synthesis and characterization of [1-13C]-and d8-arachidonic acid |
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| Authors: | Un Hoi Do M G Sundaram S Ramachandran Robert W Bryant |
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| Affiliation: | (1) Applied Science Division, Milton Roy Company, 16801 State College, Pennsylvania;(2) Department of Biochemistry, School of Medicine and Health Sciences, George, Washington University, 20037 Washington, DC |
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| Abstract: | Methyld
8- and 1-13C] 5,8,11,14-eicosatetraenoate (arachidonate) were prepared from a common synthetic precursor, 4,7,10,13-nonadecatetrayn-1-ol.
The purified products were characterized by gas chromatography-mass spectrometry. Mass spectra oft-butyldimethylsilyl esters ofd
8-and 1-13C]-arachidonic acid showed a most intense M-57]+ peak at high mass. The isotopic purity of methyl 1-13C] arachidonate was 99% and that of methyld
8-arachidonate was 56%. Whend
8-arachidonic acid was prepared by direct deuteration of 5,8,11,14-eicosatetraynoic acid, the isotopic purity of the sample
was 86%. |
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