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A droplet microfluidic approach to single-stream nucleic acid isolation and mutation detection |
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| Authors: | Dong Jin Shin Yi Zhang Tza-Huei Wang |
| Affiliation: | 1. Department of Biomedical Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, USA 2. Department of Mechanical Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, USA 3. Institute of NanoBioTechnology, Baltimore, MD, USA 4. Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD, USA |
| Abstract: | In this work, a droplet microfluidic platform for genetic mutation detection from crude biosample is described. Single-stream integration of nucleic acid isolation and amplification is realized on a simple fluidic cartridge. Subsequent DNA melting curve is employed with signal normalizing algorithm to differentiate heterozygous K-ras codon 12 c.25G>A mutant from the wild type. This technique showcases an alternative to modular bench-top approaches for genetic mutation screening, which is of interest to decentralized diagnostic platforms. |
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