WSC-DTPA纳米粒辐射防护作用的初步研究

为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站观察BRL细胞摄取FITC-WSC-DTPA纳米荧光探针的情况。结果表明:成功合成了游离氨基含量分别为92.7%、74.3%、1.59%的WSC-DTPA聚合物;WSC、WSC纳米粒以及WSC-DTPA纳米粒(氨基含量为92.7%,浓度在6.25μg/mL以上),随着药物浓度的增加,BRL细胞存活率均显著高于单纯照射组,差别有统计学意义(p<0.05),而游离氨基含量为1.59%的WSC-DTPA纳米粒无辐射保护作用;活细胞工作站检验结果显示2 h内WSC纳米粒、WSC-DTPA纳米粒能够进入BRL细胞,而非纳米化的WSC-DTPA聚合物无法进入细胞。

Study on radiation protection of WSC-DTPA nanoparticles

To investigate the radiation protection of WSC-DTPA nanoparticles, N-acylation reaction and ion crossing-linking method were used to prepare nanoparticles in different remaining free amino groups. MTT assay was performed to evaluate their effects on the viability of the cells after y-rays irradiation, while the capability of FITC-WSC-DTPA nanoparticles probe penetrating through cellular membrane was observed by Live Cell Station. The results show that WSC-DTPA polymers were successfully synthesized, in which the content free amino group is 92.7%, 74.3%, 1.59%, respectively. Compared with y-rays alone group, it was shown that WSC, WSC nanoparticles and WSC-DTPA nanoparticles with the concentration more than 6.25 μg/mL and the free amino group content around 92.7% have good efficacy in radiation protection to BRL cells from damage induced by 6 Gy 60Co y-rays by the higher survival rate, while WSC-DTPA nanoparticles with 1,59% free amino group content do not have radiation protection to BRL cells. The WSC nanoparticles and WSC-DTPA nanoparticles can penetrate into cellular within 2 h, while WSC-DTPA polymers could not pass through the membrane.