Fish Oil Normalizes Plasma Glucose Levels and Improves Liver Carbohydrate Metabolism in Rats Fed a Sucrose-Rich Diet |
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Authors: | Gustavo J Hein Adriana Chicco Yolanda B Lombardo |
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Affiliation: | (1) Department of Biochemistry, School of Biochemistry, University of Litoral, Ciudad Universitaria Paraje El Pozo. CC 242 (3000), Santa Fe, Argentina; |
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Abstract: | A sucrose-rich diet (SRD) induces insulin resistance and dyslipidemia with impaired hepatic glucose production and gluconeogenesis,
accompanied by altered post-receptor insulin signaling steps. The aim of this study was to examine the effectiveness of fish
oil (FO) to reverse or improve the impaired hepatic glucose metabolism once installed in rats fed 8 months a SRD. In the liver
of rats fed SRD in which FO replaced corn-oil during the last 2 months, as dietary fat, several key enzyme activities and
metabolites involved in glucose metabolisms (phosphorylation, glycolysis, gluconeogenesis and oxidative and non oxidative
glucose pathway) were measured. The protein mass levels of IRS-1 and αp85 PI-3K at basal conditions were also analyzed. FO
improved the altered activities of some enzymes involved in the glycolytic and oxidative pathways observed in the liver of
SRD fed rats but was unable to restore the impaired capacity of glucose phosphorylation. Moreover, FO reversed the increase
in PEPCK and G-6-Pase and reduced the G-6-Pase/GK ratio. Glycogen concentration and GSa activity returned to levels similar
to those observed in the liver of the control-fed rats. Besides, FO did not modify the altered protein mass levels of IRS-1
and αp85 PI-3K. Finally, dietary FO was effective in reversing or improving the impaired activities of several key enzymes
of hepatic carbohydrate metabolism contributing, at least in part, to the normalization of plasma glucose levels in the SRD-fed
rats. However, these positive effects of FO were not observed under basal conditions in the early steps of insulin signaling
transduction. |
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Keywords: | |
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