树脂吸附法固定Candida rugosa脂肪酶

Candida rugosa脂肪酶具有优良的催化性能,对其进行固定化可以很方便地实现酶的回收和再利用。采用南开大学化工厂生产的4种阴离子交换树脂和4种大孔吸附树脂为载体,对来源于Candida rugosa的脂肪酶进行了吸附固定化,结果表明,以大孔吸附树脂AB-8为载体的固定酶比活性最高。固定化酶制备过程中缓冲液的最适宜pH值为7.2,最佳固定化时间为1 h,载体和酶的最佳质量配比为10∶1。与游离酶相比,固定化后酶活损失大约30%,但稳定性平均约提高60%。

Immobilization of Candida rugosa lipase by adsorption on resins

To easily recycle and reuse Candida rugosa lipase with excellent catalytic properties,4 types of anionic ion exchange resins and 4 types of macroporous resins from the Chemical Factory of Nankai University were screened as the carriers to immobilize the Candida rugosa lipase by adsorption.The results show that the lipase immobilized on resin AB-8 has the highest specific activity.The optimal conditions of immobilization are buffer pH=7.2,adsorption time 1 h and mass ratio of carrier to lipase 10 ∶1.Compared with the free enzyme,the immobilized lipase loses 30% of activity,but its stability is improved by 60%.