| MALDI—MS法中基体和样品结合情况的研究 |
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| 引用本文： | 赵善楷 张金红. MALDI—MS法中基体和样品结合情况的研究[J]. 质谱学报, 1997, 18(4): 7-10 |
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| 作者姓名： | 赵善楷 张金红 |
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| 作者单位： | 中山大学测试中心!广州，5102275，中山大学测试中心!广州，5102275，中山大学测试中心!广州，5102275，中山大学测试中心!广州，5102275 |
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| 摘 要： | 本文通过静态小角激光光散方法对４种不同基体：２，５二羟基苯甲酸，咖啡酸，尼古丁酸和３－氢基－４羟基苯甲酸与蛋白质，溶菌酶和细菌色素Ｃ在水或水与乙腈溶液中的行为进行了观察，结果发现３－氨基－４羟基苯甲酸分别在上述两种蛋白质的行为进行了观察。
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| 关 键 词： | 基体辅助 激光解吸电离 质谱法 蛋白质 |
A Study on Matrix and Protein Samples Association in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry |
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| Zhao Shankai,Zhang Jinghong,Zhu Zhihua,Zha Qingmin. A Study on Matrix and Protein Samples Association in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 1997, 18(4): 7-10 |
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| Authors: | Zhao Shankai Zhang Jinghong Zhu Zhihua Zha Qingmin |
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| Abstract: | By the use of low-angle laser light scattering photometer, the associateon mannerof matrix and protein in water or water acetonitrile solution was investigated. The matrices studied were 2, 5-dihydroxybenzoic acid, caf feic acid, nicotinic acid and 3-amino-4-hydroxybenzoic acid and the proteins were lysozyme and cytochrome C. The resultsdemonstrated that the apparent molecular weight and the second variel coefficient A2 insolution of 3-amino-4-hydroxybenzoic acid with lysozyme of cytochrome C were at leastthree time higher than that in the otherthree matrices solution. It means that in the solution, 3-amino-4-hydroxybenzoic acid associated very strongly with these two proteinsReferring to the effects on matrix-assisted laser desorption/ionization mass spectrometry,the above results were discussed. |
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| Keywords: | laser light scattering matrix-assisted laser desorption/ionization mass spectrometry protein |
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