Efficient construction of cDNA microarrays utilizing normalized cDNA libraries of Arabidopsis thaliana |
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| Authors: | Ando Kohei Yamakawa Seiyei Miyashita Kyoko Yoshida Kazuya Yokota Akiho Shinmyo Atsuhiko Kohchi Takayuki |
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| Affiliation: | Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan. |
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| Abstract: | We have demonstrated the utility of normalization for efficient cDNA microarray preparation using Arabidopsis as a model. Nonredundant cDNAs including 5722 species were efficiently collected from random 7914 expressed sequence tags (ESTs) in four normalized cDNA libraries. The prepared microarrays were successfully used to monitor gene expression. These methodologies should be applicable to the study of other species in plant biotechnology. |
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