Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice |
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Authors: | Leiko Asakura Patrícia M Cazita Lila M Harada Valéria S Nunes Jairo A Berti Alessandro G Salerno Daniel F J Ketelhuth Magnus Gidlund Helena C F Oliveria Eder C R Quintão |
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Affiliation: | 1. Lipids Lab, University of S?o Paulo Medical School, S?o Paulo, Brazil 2. Department of Physiology and Biophysics, Biology Institute at State University of Campinas (UNICAMP), S?o Paulo, Brazil 3. Department of Immunology, Biomedical Sciences Institute at the University of S?o Paulo, Brazil
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Abstract: | The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been
investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr+/− CETP+/−). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental
groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n=25), or at high-dose (Iso High, 0.535, n=28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n=28). Aortic root lipid-stained lesion area (mean μm2×103±SD) did not differ among Iso Low (12.3±9.9), Iso High (7.4±6.4), and controls (10.7±12.8). Autoantibody titers against plasma
oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso
Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human 4-14C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained
area in the aortic root. |
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